The majority of chronic mental illnesses (CMI) involve risk factors that contribute to the illness’s onset and do not have a single underlying cause. No specific singular cause or mechanism for the development of mental illnesses have been established yet. Prior studies have revealed the role of protein aggregation as a potential cause of chronic mental illness. CRMP1 is one of the most recently found and least studied proteins whose aggregation is associated with mental illness, particularly schizophrenia. Different versions of the CRMP1 Lv isoform, with deletions of different parts of N-terminal region were explored in this research to determine the influence of N-terminal region on aggregation. CRMP1 isoforms were expressed in vitro using HEK293 and SH-SY5Y cells and examined using fluorescent microscopy and a quantitative blinded test assay. While previous studies have suggested CRMP1 Lv to be more prone to aggregation than CRMP1 Sv, in this more relevant and through experiment both CRMP1 Lv and CRMP1 Sv appear to have similar levels of aggregation. Compared to other studies, a relatively small, FLAG tag is used in this research, which is less likely to obstruct expression and function of protein than GFP fusion proteins used previously, and using human neuroblastoma cells SH-SY5Y provides a more realistic setting than in prior research. Although the results were not statistically significant, they do suggest that the unique N-terminus region of CRMP1 Lv may be required for its aggregation. Thus, further research is needed, and it should include examining the effects of proteasome inhibitor and/or introduction reactive oxygen species triggering the cell stress. Co-aggregation with DISC1 should also be investigated further with different isoforms of CRMP1 Lv