Behavioral sensitization represents non-associative learning in which repeated administration of stimulus results in a progressive increase in response. Behavioral sensitization in Drosophila can be short-term (takes less than 30 minutes) or long-term (lasts up to two days). It is assumed that they rely on different molecular mechanisms. The aim of the study was the development of high-throughput method to develop long-term behavioral sensitization to psychostimulants cocaine and methamphetamine. All tests were performed on male wild type (wt) Drosophila melanogaster, of strain Canton S, and mutants period (per) and timeless (tim), of which is per previously associated with the development of behavioral sensitization, while tim is not. Oral administration of methamphetamine in flies induce tolerance, regardless of the day time, concentration or duration of administration. As opposed to that, the administration of volatilized cocaine or methamphetamine led to a rapid increase in locomotor activity which was dose dependent (75-1.25ěl) and time dependent (1 to 7 minute length). For administration of volatilized psychostimulants we have developed a new method where with regulation of airflow volatilized substance is delivered to individual flies in DAMS (Drosophila Activity Monitoring System). The next experiments will define requirements in this high-throughput system for induction of behavioral sensitization to cocaine and methamphetamine. Also, we developed a method for the induction of short-term bihevioral sensitization to mechanical stress. Flies were exposed to an initial strong mechanical stress (startle, ST), and then a series of weaker sensitizing stimulus (SS1-3). Flies are more responsive to the SS stimulus if they were previously treated with ST, which is in accordance with previously published results. On ST and SS per and tim flies react like wt which shows for the first time that these genes are not involved in the regulation of short-term bihevioral sensitization, and indicate the differences in the mechanisms of induction of short and long-term behavioral sensitization. Our results are in line with dual-process theory of plasticity response to repeated stimulation by Groves and Thompson 1970. which tells that development of sensitizaion or habituation depends on the strength and duration of the stimulus. Oral administration (long-terme stimulus od low intensity) caused tolerance, while the mechanical stress (short-term stimulus of high intensity) induced development of behavioraln sensitization. The characteristic of the stimulus given by volatilized cocaine satisfies conditions for the development of behavioral sensitzation which will be implemented in the next experiments.