Life expectancy has increased dramatically over the past few decades worldwide, with an increase in the occurrence of numerous chronic diseases. Aging is a complex process that involves a progressive decrease in tissue function and loss of regenerative capacity, underlined by cellular senescence, a stable state of cell cycle arrest that occurs in response to damage and accumulation of cellular stress. Aging increases the frequency of occurrence of senescent cells, and for this reason, various anti-aging strategies have been developed in recent years. In this work, I wanted to show that by changing the ratio of young vs. old cells in the skin of Fischer 344 rats, we can change the tissue microenvironment and a reduce the effect of senescence-associated secretory phenotype (SASP). The obtained results show that the micro-transplantation of young cells in the skin of rats has a positive effect on the revitalization of the niche of stem cells and an increase in the total number of cells in the tissue, and at the same time I have demonstrated that this effect persist for longer period of time. Changes in gene expression in skin tissue with aging, or changes as a result of treatment, were also shown. In conclusion, I have shown that I successfully reduced the secretory phenotype of senescent cells, changed the homeostasis of old tissue and successfully regenerated the skin.