Nontuberculous Mycobacteria (NTM) is a heterogeneous group of environmental bacteria most commonly isolated from water and soil. Even though NTM have low pathogenic potential, they are still capable of causing numerous diseases in humans. Potential sources of infection consist of drinking water, water tanks, sea water, contaminated showerheads, aerosols and dust. NTM infections in 80-90% of cases manifest as pulmonary infections (2,5). The main goal of this paper was to test the susceptibility of nontuberculous mycobacteria M. intracellulare (ATCC 13950) to disinfectants widely used in medicinal practice. The disinfectants / antiseptics whose effectiveness was tested were Descocid - N (2,5%), Manorapid and 70% ethyl alcohol. The subcultivation of the tested strain was carried out on an enriched Middlebrook 7H10 growth medium in aerobic atmospheric conditions, at the temperature 37°C for 3 weeks. The suspension test did not show any growth of the tested strain on the Middlebrook 7H10 growth medium after the application of 70% ethyl alcohol and Manorapid solution. Therefore, the microbicidal effect of these disinfectants on M. intracellulare within contact times of 1, 5 and 30 minutes was confirmed. Descocid – N did not have aforementioned microbicidal effect on M. intracellulare with regardless of the contact times (1, 5 and 30 minutes). Although, 2,5% solution of Descocid - N did reduce the number of bacteria in the suspension dependent on contact time.