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Synthesis, characterisation and in vitro investigation of photodynamic activity of 5-(4- octadecanamidophenyl)-10, 15, 20-tris(N- methylpyridinium-3-yl)porphyrin trichloride on HeLa cells using low light fluence rate
Photodiagnosis and Photodynamic Therapy, 15 (2016); 115-126. https://doi.org/10.1016/j.pdpdt.2016.07.003
Malatesti, Nela; Harej, Anja; Kraljević Pavelić, Sandra; Lončarić, Martin; Zorc, Hrvoje; Wittine, Karlo; Anđelković, Uroš; Josić, Đuro

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Malatesti, N., Harej, A., Kraljević Pavelić, S., Lončarić, M., Zorc, H., Wittine, K. ... Josić, Đ. (2016). Synthesis, characterisation and in vitro investigation of photodynamic activity of 5-(4- octadecanamidophenyl)-10, 15, 20-tris(N- methylpyridinium-3-yl)porphyrin trichloride on HeLa cells using low light fluence rate. Photodiagnosis and Photodynamic Therapy, 15., 115-126. doi: 10.1016/j.pdpdt.2016.07.003

Malatesti, Nela, et al. "Synthesis, characterisation and in vitro investigation of photodynamic activity of 5-(4- octadecanamidophenyl)-10, 15, 20-tris(N- methylpyridinium-3-yl)porphyrin trichloride on HeLa cells using low light fluence rate." Photodiagnosis and Photodynamic Therapy, vol. 15, 2016, str. 115-126. https://doi.org/10.1016/j.pdpdt.2016.07.003

Malatesti, Nela, Anja Harej, Sandra Kraljević Pavelić, Martin Lončarić, Hrvoje Zorc, Karlo Wittine, Uroš Anđelković i Đuro Josić. "Synthesis, characterisation and in vitro investigation of photodynamic activity of 5-(4- octadecanamidophenyl)-10, 15, 20-tris(N- methylpyridinium-3-yl)porphyrin trichloride on HeLa cells using low light fluence rate." Photodiagnosis and Photodynamic Therapy 15 (2016): 115-126. https://doi.org/10.1016/j.pdpdt.2016.07.003

Malatesti, N., et al. (2016) 'Synthesis, characterisation and in vitro investigation of photodynamic activity of 5-(4- octadecanamidophenyl)-10, 15, 20-tris(N- methylpyridinium-3-yl)porphyrin trichloride on HeLa cells using low light fluence rate', Photodiagnosis and Photodynamic Therapy, 15, str. 115-126. doi: 10.1016/j.pdpdt.2016.07.003

Malatesti N, Harej A, Kraljević Pavelić S, Lončarić M, Zorc H, Wittine K, i sur.. Synthesis, characterisation and in vitro investigation of photodynamic activity of 5-(4- octadecanamidophenyl)-10, 15, 20-tris(N- methylpyridinium-3-yl)porphyrin trichloride on HeLa cells using low light fluence rate. Photodiagnosis and Photodynamic Therapy [Internet]. 2016. [pristupljeno 24.04.2024.];15:115-126. doi: 10.1016/j.pdpdt.2016.07.003

N. Malatesti, et al., "Synthesis, characterisation and in vitro investigation of photodynamic activity of 5-(4- octadecanamidophenyl)-10, 15, 20-tris(N- methylpyridinium-3-yl)porphyrin trichloride on HeLa cells using low light fluence rate", Photodiagnosis and Photodynamic Therapy, vol. 15, str. 115-126, 2016. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:193:798368. [Citirano: 24.04.2024.]

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Naslov (engleski)Synthesis, characterisation and in vitro investigation of photodynamic activity of 5-(4- octadecanamidophenyl)-10, 15, 20-tris(N- methylpyridinium-3-yl)porphyrin trichloride on HeLa cells using low light fluence rate
AutorNela Malatesti
AutorAnja Harej
AutorSandra Kraljević Pavelić
AutorMartin Lončarić
AutorHrvoje Zorc
AutorKarlo Wittine
AutorUroš Anđelković
AutorĐuro Josić
Autorova ustanovaSveučilište u Rijeci
(Fakultet biotehnologije i razvoja lijekova)
Znanstveno / umjetničko
područje, polje i grana		INTERDISCIPLINARNA PODRUČJA ZNANOSTI
Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)
Sažetak (engleski)
Photodynamic therapy (PDT) is a treatment that aims to kill cancer cells by reactive oxygen species, mainly singlet oxygen, produced through light activation of a photosensitiser (PS). Amongst photosensitisers that attracted the most attention in the last decade are cationic and amphiphilic molecules based on porphyrin, chlorin and phthalocyanine structures. Our aim was to join this search for more optimal balance of the lipophilic and hydrophilic moieties in a PS. A new amphiphilic porphyrin, 5-(4-octadecanamidophenyl)-10, 15, 20- tris(N-methylpyridinium-3-yl)porphyrin trichloride (5) was synthesised and characterised by 1H NMR, UV–vis and fluorescence spectroscopy, and by MALDI-TOF/TOF spectrometry. In vitro photodynamic activity of 5 was evaluated on HeLa cell lines and compared to the activity of the hydrophilic 5-(4- acetamidophenyl)-10, 15, 20-tris(N- methylpyridinium-3-yl)porphyrin trichloride (7). Low fluence rate (2 mW cm−2) of red light (643 nm) was used for the activation, and both porphyrins showed a drug dose-response as well as a light dose-response relationship, but the amphiphilic porphyrin was presented with significantly lower IC50 values. The obtained IC50 values for 5 were 1.4 μM at 15 min irradiation time and 0.7 μM when the time of irradiation was 30 min, while for 7 these values were 37 and 6 times higher, respectively. These results confirm the importance of the lipophilic component in a PS and show a potential for 5 to be used as a PS in PDT applications.
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran - međunarodna recenzija
Verzija publikacijeRukopis poslan na recenzijski postupak (preprint)
Naslov časopisaPhotodiagnosis and Photodynamic Therapy
Brojčani podacivol. 15, str. 115-126
p-ISSN1572-1000
DOIhttps://doi.org/10.1016/j.pdpdt.2016.07.003
URN:NBNurn:nbn:hr:193:798368
Datum objave publikacije2016
URL dokumentahttp://bib.irb.hr/830506
Vrsta resursaTekst
Prava pristupaOgraničen pristup
Uvjeti korištenjaIkona licence Zaštićeno autorskim pravom.
Datum i vrijeme pohrane2018-09-13 15:42:10