The use of psychostimulants such as methamphetamine leads to addiction and causes negative consequences for the organisms. With repeated use of the same dose of psychostimulants, locomotor activity increases and locomotor sensitization occurs. Drosophila melanogaster is a well-known model for research on addiction and the negative effects of psychostimulants on the body. Psychostimulants affect the locomotor activity and circadian rhythm of fruit flies, and when psychostimulants are stopped suddenly withdrawal symptoms occur. This research aimed to examine whether there are long-term effects on the activity of flies treated with two doses of volatility methamphetamine and how lighting affect this. We were interested in the difference in locomotor activity between controls, flies that developed locomotor sensitization, and those that did not develop locomotor sensitization after administration of two doses of vMETH, during 12h light: 12h dark and continuous darkness conditions. The obtained results indicate that in the experiment conducted in constant darkness, flies that have develop LS have higher activity compared to flies that were kept under conditions of 12h light:12h dark, while flies that did not develop locomotor sensitization had lower activity compared to the control group and flies that developed locomotor sensitization. Further research is needed to better understand the long-term effects of volatilized methamphetamine on flies, of which locomotor activity can be one of the markers of withdrawal symptoms.