prikaz prve stranice dokumenta Proteomic analysis of opossum Monodelphis domestica spinal cord reveals the changes of proteins related to neurodegenerative diseases during developmental period when neuroregeneration stops being possible
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Proteomic analysis of opossum Monodelphis domestica spinal cord reveals the changes of proteins related to neurodegenerative diseases during developmental period when neuroregeneration stops being possible
Biochemical and Biophysical Research Communications, 587 (2021); 85-91. https://doi.org/10.1016/j.bbrc.2021.11.078
Tomljanović, Ivana; Petrović, Antonela; Ban, Jelena; Mladinic, Miranda

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Tomljanović, I., Petrović, A., Ban, J. i Mladinic, M. (2021). Proteomic analysis of opossum Monodelphis domestica spinal cord reveals the changes of proteins related to neurodegenerative diseases during developmental period when neuroregeneration stops being possible. Biochemical and Biophysical Research Communications, 587., 85-91. doi: 10.1016/j.bbrc.2021.11.078

Tomljanović, Ivana, et al. "Proteomic analysis of opossum Monodelphis domestica spinal cord reveals the changes of proteins related to neurodegenerative diseases during developmental period when neuroregeneration stops being possible." Biochemical and Biophysical Research Communications, vol. 587, 2021, str. 85-91. https://doi.org/10.1016/j.bbrc.2021.11.078

Tomljanović, Ivana, Antonela Petrović, Jelena Ban i Miranda Mladinic. "Proteomic analysis of opossum Monodelphis domestica spinal cord reveals the changes of proteins related to neurodegenerative diseases during developmental period when neuroregeneration stops being possible." Biochemical and Biophysical Research Communications 587 (2021): 85-91. https://doi.org/10.1016/j.bbrc.2021.11.078

Tomljanović, I., et al. (2021) 'Proteomic analysis of opossum Monodelphis domestica spinal cord reveals the changes of proteins related to neurodegenerative diseases during developmental period when neuroregeneration stops being possible', Biochemical and Biophysical Research Communications, 587, str. 85-91. doi: 10.1016/j.bbrc.2021.11.078

Tomljanović I, Petrović A, Ban J, Mladinic M. Proteomic analysis of opossum Monodelphis domestica spinal cord reveals the changes of proteins related to neurodegenerative diseases during developmental period when neuroregeneration stops being possible. Biochemical and Biophysical Research Communications [Internet]. 27.11.2021. [pristupljeno 26.04.2024.];587:85-91. doi: 10.1016/j.bbrc.2021.11.078

I. Tomljanović, A. Petrović, J. Ban i M. Mladinic, "Proteomic analysis of opossum Monodelphis domestica spinal cord reveals the changes of proteins related to neurodegenerative diseases during developmental period when neuroregeneration stops being possible", Biochemical and Biophysical Research Communications, vol. 587, str. 85-91, Studeni 2021. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:193:022227. [Citirano: 26.04.2024.]

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Podaci o radu
Naslov (engleski)Proteomic analysis of opossum Monodelphis domestica spinal cord reveals the changes of proteins related to neurodegenerative diseases during developmental period when neuroregeneration stops being possible
AutorIvana Tomljanović
AutorAntonela Petrović
AutorJelena Ban
AutorMiranda Mladinic
Autorova ustanovaSveučilište u Rijeci
(Fakultet biotehnologije i razvoja lijekova)
Znanstveno / umjetničko
područje, polje i grana		INTERDISCIPLINARNA PODRUČJA ZNANOSTI
Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)
Sažetak (engleski)
One of the major challenges of modern neurobiology concerns the inability of the adult mammalian central nervous system (CNS) to regenerate and repair itself after injury. It is still unclear why the ability to regenerate CNS is lost during evolution and development and why it becomes very limited in adult mammals. A convenient model to study cellular and molecular basis of this loss is neonatal opossum (Monodelphis domestica). Opossums are marsupials that are born very immature with the unique possibility to successfully regenerate postnatal spinal cord after injury in the first two weeks of their life, after which this ability abbruptly stops. Using comparative proteomic approach we identified the proteins that are differentially distributed in opossum spinal tissue that can and cannot regenerate after injury, among which stand out the proteins related to neurodegenerative diseases (NDD), such as Huntington, Parkinson and Alzheimer's disease, previously detected by comparative transcriptomics on the analog tissue. The different distribution of the selected proteins detected by comparative proteomics was further confirmed by Western blot (WB), and the changes in the expression of related genes were analysed by quantitative reverse transcription PCR (qRT-PCR). Furthermore, we explored the cellular localization of the selected proteins using immunofluorescent microscopy. To our knowledge, this is the first report on proteins differentially present in developing, non-injured mammalian spinal cord tissue with different regenerative capacities. The results of this study indicate that the proteins known to have an important role in the pathophysiology of neurodegeneration in aged CNS, could also have an important phyisological role during CNS postnatal development and in neuroregeneration process.
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran - međunarodna recenzija
Verzija publikacijeObjavljena verzija rada (izdavačev PDF)
Naslov časopisaBiochemical and Biophysical Research Communications
Brojčani podacivol. 587, str. 85-91
p-ISSN0006-291X
DOIhttps://doi.org/10.1016/j.bbrc.2021.11.078
URN:NBNurn:nbn:hr:193:022227
Datum objave elektroničkog izdanja2021-11-27
ProjektŠifra: HRZZ-IP-2016-06-7060
Naziv (hrvatski): Istraživanje granice između neurodegeneracije i neuroregeneracije: identifikacija ključnih molekula pomoću proteomike i funkcionalnih testova na leđnoj moždini sisavaca
Kratica: DefineREgenAgeMode
Voditelj: Miranda Mladinić Pejatović
Pravna nadležnost: Hrvatska
Financijer: HRZZ
URL dokumentahttps://www.sciencedirect.com/science/article/pii/S0006291X21016041?via%3Dihub
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
Uvjeti korištenjaIkona licence Imenovanje 4.0 međunarodna (CC BY 4.0)
Datum i vrijeme pohrane2022-02-02 20:10:22