Nutrition is one of the most important factors for the growth and development of the human life and for health maintenance. Proper nutrition of preschool children is important because of their accelerated growth and development and should be based on a nutritional pyramid. It implies nutrients, represented and consumed in a balanced ratio. Vitamins are substances of organic origin that the body needs in relatively small quantities to grow, to maintain health and its metabolic integrity. They are ingested through diet or dietary supplements in cases of insufficient synthesis in the human body. There are 13 known vitamins that are divided according to their solubility into fat-soluble vitamins (vitamins A, D, E and K) and water-soluble ones (B vitamins and vitamin C). Recommended daily intake doses are necessary to be met because a deficiency of any of the aforementioned vitamins in the body causes a syndrome with typical signs and symptoms. The aim of the study was to measure the values of vitamins A, B1, B2, B6 and vitamin C from whole-day meals sampled in kindergartens and compare them with the recommendations for preschool age children. According to the concluded contracts, sampling of whole-day meals is done four times a year, by method of random samples. The samples are homogenized and preserved until saponification, derivatization and final determination by high performance liquid chromatography (HPLC). Average daily intakes of vitamins A, B1, B2 and C in the period of 10 years of observation did not meet 80% of the recommendations each year, while vitamin B6 is continuously above the recommendations of the Food Standard for diet planning in kindergartens. Monitoring the vitamin content in children's whole-day meals is important because of the timely response to possible deficiencies and inadequate intake. When planning the menu, focus should be on fresh, seasonal fruits and vegetables that do not require further heat treatment and are naturally rich in micronutrients. It is important to educate parents and kindergarten professionals about healthy and proper nutrition and the benefits of healthy habits acquired in childhood.