prikaz prve stranice dokumenta Differentiation of acute and chronic exposure to METH on fAGEs accumulation and behavioral phenotypes
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master's thesis
Differentiation of acute and chronic exposure to METH on fAGEs accumulation and behavioral phenotypes
2023. urn:nbn:hr:193:882355
Klasan, Ana
University of Rijeka
Faculty of Biotechnology and Drug Development

Cite this document

Klasan, A. (2023). Differentiation of acute and chronic exposure to METH on fAGEs accumulation and behavioral phenotypes (Master's thesis). Rijeka: University of Rijeka. Retrieved from https://urn.nsk.hr/urn:nbn:hr:193:882355

Klasan, Ana. "Differentiation of acute and chronic exposure to METH on fAGEs accumulation and behavioral phenotypes." Master's thesis, University of Rijeka, 2023. https://urn.nsk.hr/urn:nbn:hr:193:882355

Klasan, Ana. "Differentiation of acute and chronic exposure to METH on fAGEs accumulation and behavioral phenotypes." Master's thesis, University of Rijeka, 2023. https://urn.nsk.hr/urn:nbn:hr:193:882355

Klasan, A. (2023). 'Differentiation of acute and chronic exposure to METH on fAGEs accumulation and behavioral phenotypes', Master's thesis, University of Rijeka, accessed 24 April 2024, https://urn.nsk.hr/urn:nbn:hr:193:882355

Klasan A. Differentiation of acute and chronic exposure to METH on fAGEs accumulation and behavioral phenotypes [Master's thesis]. Rijeka: University of Rijeka; 2023 [cited 2024 April 24] Available at: https://urn.nsk.hr/urn:nbn:hr:193:882355

A. Klasan, "Differentiation of acute and chronic exposure to METH on fAGEs accumulation and behavioral phenotypes", Master's thesis, University of Rijeka, Rijeka, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:193:882355

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Metadata
TitleDifferentiation of acute and chronic exposure to METH on fAGEs accumulation and behavioral phenotypes
Title (croatian)Diferencijacija akutnog i kroničnog izlaganja METH-u na akumulaciju fAGEs te bihevioralni fenotip
AuthorAna Klasan
MentorRozi Andretić Waldowski (mentor)
Committee memberRozi Andretić Waldowski (član povjerenstva)
Committee memberMiranda Mladinić Pejatović (predsjednik povjerenstva)
Committee memberMladen Merćep (član povjerenstva)
Committee memberJelena Ban (član povjerenstva)
GranterUniversity of Rijeka
(Faculty of Biotechnology and Drug Development)
Rijeka
Defense date and country2023-09-27, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Methamphetamine (METH) and cocaine (COC) are often used recreational drugs that target dopaminergic synaptic transmission. Repeated exposure to a specific drug that causes increased motor-stimulant response is termed behavioral sensitization. In D. melanogaster, behavioral sensitization can be measured and induced with METH and COC. Dopamine has a role in changes included in neural plasticity after drug intake, such as behavioral sensitization. Furthermore, it determines various behaviors, including amount of sleep and locomotor activity. It increases levels of oxidative stress and affects accumulation of fluorescent advanced glycation end products (fAGEs). Based on these findings, we were interested to explore how different duration of feeding with METH affects motor activating effects of volatilized METH (vMETH) and volatilized cocaine (vCOC), amount of locomotor activity and sleep, and biochemical indicators of oxidative stress such as fAGEs and hydrogen peroxide (H2O2). Feeding with METH did not influence changes in locomotor activity after vCOC and vMETH administration using FlyBong. However, we did observe that locomotor activity and response to psychostimulants decreases with age. Furthermore, results suggest that neural changes caused by METH are governed by different mechanisms to those that control neural changes influenced by COC. Flies pretreated with METH exhibited decreased activity and increased sleep through the period of fourteen days. Furthermore, we measured levels of fAGEs and H2O2 in the head of flies. Measurements showed decrease of H2O2 and increase of fAGEs through the period of fourteen days. This indicates possible activation of antioxidative defense which decreased H2O2 levels. Increase of fAGEs through fourteen days confirmed its role as indicators of ageing in D. melanogaster. Further studies are needed to elucidate the exact effect of oral METH feeding on behavioral and biochemical factors in D. melanogaster.
Abstract (croatian)
Metamfetamin (METH) i kokain (COC) su često konzumirane rekreativne droge, čiji mehanizam utječe na sinaptičku transmisiju dopamina. Uzastopno izlaganje specifičnoj drogi uzrokuje povećani motorički stimulirani odgovor kojeg se naziva bihevioralna senzitacija. METH i COC mogu inducirati bihevioralnu senzitaciju kod D. melanogaster. Dopamin ima ulogu u promjenama vezane uz neuralnu plastičnost, kao što je bihevioralna senzitacija, koje nastupaju nakon uzimanja droge. Također, ima ulogu u regulaciji količine spavanja, lokomotorne aktivnosti te utječe na akumulaciju fAGEs-a i količinu oksidativnog stresa. Uzevši u obzir prethodne činjenice, u ovome radu smo željeli istražiti kako različita dužina oralnog hranjenja METH-om utječe na motorno-aktivirajuće efekte volatiliziranog METH-a (vMETH) i volatiliziranog kokaina (vCOC), količinu lokomotorne aktivnosti i spavanja, te biokemijske indikatore oksidativnog stresa fAGEs i vodikovog peroksida (H2O2). Hranjenje METH-om nije utjecalo na promjene u lokomotornoj aktivnosti nakon administracije vCOC i vMETH putem FlyBong platforme. Međutim, uočili smo da lokomotorna aktivnost i odgovor mušica na psihostimulante opadaju sa starosti. Rezultati sugeriraju da su neurološke promjene koje uzrokuje METH regulirane mehanizmom koji nije povezan s mehanizmom koji kontrolira neurološke promjene nakon administracije COC-a. Nadalje, mušice koje su hranjenje METH hranom pokazale su smanjenu aktivnost i povećano spavanje kroz četrnaest dana. Nadalje, mjerili smo koncentraciju fAGEs-a i H2O2 u glavama mušica. Mjerenja su pokazala opadanje koncentracije H2O2 i porast koncentracije fAGEs-a kroz četrnaest dana. Pad u koncentraciji H2O2 možemo opravdati aktivacijom antioksidativne obrane organizma nakon izlaganja METH-u. Povećanje koncentracije fAGEs-a daje potvrdu prethodnim istraživanjima koja sugeriraju da je fAGEs indikator starosti mušica. Potrebno je još studija da bi se saznao točan efekt oralnog hranjenja METH-om na bihevioralne i biokemijske faktore kod D. melanogaster.
Keywords
Keywords (croatian)
Languageenglish
URN:NBNurn:nbn:hr:193:882355
Study programmeTitle: Drug research and development
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra istraživanja i razvoja lijekova (magistar/magistra istraživanja i razvoja lijekova)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2023-10-11 10:22:52