Title Učinci umjerene konzumacije bijelog vina na vaskularnu reaktivnost izolirane torakalne aorte štakora
Title (english) The effects of moderate white wine consumption on vascular reactivity of the isolated rat thoracic aorta
Author Sara Srzić
Mentor Ivana Mudnić (mentor)
Mentor Miranda Mladinić Pejatović (komentor)
Committee member Ivana Mudnić (predsjednik povjerenstva)
Committee member Miranda Mladinić Pejatović (član povjerenstva)
Committee member Ivana Ratkaj (član povjerenstva)
Committee member Jelena Ban (član povjerenstva)
Granter University of Rijeka (Faculty of Biotechnology and Drug Development) Rijeka
Defense date and country 2022-08-02, Croatia
Scientific / art field, discipline and subdiscipline BIOTECHNICAL SCIENCES Biotechnology
Abstract Rezultati epidemioloških studija jasno ukazuju na povoljne učinke umjerene
konzumacije vina na ljudsko zdravlje, posebice na kardiovaskularni sustav.
Vino usporava razvoj ateroskleroze i arterioskleroze ostvarujući
protuupalne, antitrombocitne i antioksidacijske učinke. Osim alkohola,
vinski polifenoli smatraju se posrednicima ovih učinaka. Tradicionalna
macerirana bijela vina s bogatim fenolnim sadržajem privlače pozornost
proizvođača i znanstvenika, a njihovi biološki učinci slabije su istraženi. Cilj
provedenog istraživanja bio je ispitati i usporediti utjecaj umjerene
konzumacije standardnog i maceriranog bijelog vina na vazodilatacijski i
vazokonstrikcijski odgovor aorte štakora.
Sprague Dawley štakori, mužjaci (N = 45) randomizirani su u 3 skupine i
podvrgnuti 4-tjednom konzumacijskom protokolu: kontrolna (K) skupina
konzumira isključivo vodu, skupina SV standardno, a skupina MV
macerirano bijelo vino. Nakon konzumacije slijedi izolacija torakalne aorte
štakora i preparacija vaskularnih prstenova. Prstenovi svih skupina (N =
107) dodatno se randomiziraju i izlažu jednom od dva doza – učinak
protokola u in vitro uvijetima: vazodilatacijski gdje su prekontrahirani
prstenovi tretiraju kumulativnim koncentracijama acetilkolina; i
vazkonstrikcijski, bez prekontrakcije, s kumulativnim koncentracijama
noradrenalina. Raspon je primijenjenih koncentracija acetilkolina i
noradrenalina jednak s konačnim koncentracijama u organskom bazenčiću
od 1nM do 10 µM). Glavne mjere ishoda su maksimalni vazodilatacijski i
vazokonstrikcijski učinak (Emax) te koncentracija potrebna za postizanje 50
% maksimalnog učinka (EC50).
Vazokonstrikcijski učinak nije se razlikovao među skupinama. Međutim,
konzumacija maceriranog bijelog vina promijenila je vazodiatacijsku
učinkovitost acetilkolina koji je u koncentracijskom rasponu od 0,1 do 1 µM
ostvario značajno veći učinak na vaskularnim prstenovima aorta MV
štakora. Sukladno, potentnost je acetilkolina bila najveća u MV, nešto
manja u SV, a najmanja u K skupini s pripadajućim EC50 vrijednostima od
43 nM, 57 nM i 70 nM za MV, SV i K.
Abstract (english) The results of epidemiological studies clearly indicate the beneficial effects
of moderate wine consumption on human health, especially on the
cardiovascular system. Wine slows the development of atherosclerosis and
arteriosclerosis by achieving anti-inflammatory, antiplatelet and antioxidant
effects. Except alcohol, wine polyphenols are thought to mediate these
effects. Traditional macerated white wines with rich phenolic content attract
the attention of producers and scientists, and their biological effects are less
well examined. The aim of the study was to examine and compare the
impact of moderate consumption of standard and macerated white wine on
the vasodilation and vasoconstriction response of the rat aorta.
Male Sprague Dawley rats (N = 45) were randomized into 3 groups and
subjected to a 4-week consumption protocol: the control (K) group
consumed only water, the SV group standard, and the MV macerated white
wine. Following consumption trial, the rat thoracic aortas were isolated and
the vascular rings prepared. The rings of all groups (N = 107) were
additionally randomized and exposed to one of two in vitro dose - response
protocols: vasodilatory protocol where the precontracted rings were treated
with acetylcholine and vasoconstriction protocol, without precontraction
with noradrenaline. Acetylcholine and noradrenaline were applied
cumulatively to acomplish final concentrations from 1nM to 10 µM in the
organ baths. The main outcome measures were the maximal vasodilation
and vasoconstriction effect (Emax) and the concentration of noradrenaline
and acetylcholine that produce 50% of maximal effect (EC50).
Noradrenaline-induced vasoconstriction effect was similar for all groups.
However, the consumption of macerated white wine changed the
vasodilation efficacy of acetylcholine, which induced a significantly greater
vasodilatory effect of MV rat aorta in the concentration range from 0.1 to 1
µM. Accordingly, the potency of acetylcholine was the highest in MV, slightly
lower in SV, and the lowest in the K group with corresponding EC50 values
of 43 nM, 57 nM and 70 nM for MV, SV and K, respectively.
The obtained results indicate a vasoprotective effect of moderate
consumption of macerated white wine in rats. The effect of standard white
wine consumption needs to be further clarified.
Keywords
Kardiovaskularni sustav
standardno bijelo vino
macerirano bijelo vino
vaskularni prstenovi
acetilkolin
noradrenalin
vazodilatacijski učinak
vazokonstricijski učinak
štakor
Keywords (english)
Cardiovascular system
standard white wine
macerated white wine
vascular rings
acetylcholine
noradrenaline
vasodilation effect
vasoconstriction effect
rat
Language croatian
URN:NBN urn:nbn:hr:193:261774
Study programme Title: Biotechnology in medicine Study programme type: university Study level: graduate Academic / professional title: magistar/magistra biotehnologije u medicini (magistar/magistra biotehnologije u medicini)
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Created on 2022-10-24 11:18:16