Allergic reactions (hypersensitivity reactions) represent a significant medical problem of modern society and lifestyle. Of particular importance are respiratory allergic reactions in children, which are increasingly becoming chronic or progressing into asthma, and allergic reactions occuring as occupational diseases. One of the main goals in scientific research of allergies is finding new diagnostic tools for allergic diseases as well as new possibilities for specific immunotherapy. For this graduate thesis nanolipid formulations of proteins isolated from the Ambrosia elatior pollen were prepared and characterized. The formulations are based on liposomes with embedded proteins that are incorporated into the gel network of the peptide hydrogelator Ac-L-Phe-L-Phe-L-Ala-NH2. Multilamellar liposomes were prepared using the method of thin lipid film hydration. Efficacy of incorporating A. elatior proteins into liposomes was spectrophotometrically determined to be approx. 13.01%. Prepared liposomal formulations of the allergens were characterized by dynamic light scattering method. It was demonstrated that incorporation of A. elatior proteins leads to an increase in liposomes and an increase in negative surface charge. The gelling properties of the hydrogelator Ac-L-Phe-L-Phe-L-Ala-NH2 with addition of liposomes were also studied. It was shown that liposomes do not impair the gel network of hydrogelator at lipid concentrations lower than the gelator concentration. The incorporation of Ambrosia elatior proteins and model protein albumin (BSA) / FITC-BSA resulted in slower gel formation or inability to transition into gel. BSA and FITC-BSA liposome formulations incorporated in the hydrogel were characterized by electron (TEM) and confocal microscopy. Transmission electron microscopy confirmed the incorporation of liposomes into the hydrogel. In gel samples with built-in BSA, the gel network preserved integrity, but it was noticed that the gels fiber were thinner and thicker. Confocal microscopy proved the incorporation of FITC-BSA into liposomes in the hydrogel.