prikaz prve stranice dokumenta PROBIR MODULATORA GAMA SEKRETAZE U LJUDSKIM STANICAMA NEUROOBLASTOMA
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master's thesis
PROBIR MODULATORA GAMA SEKRETAZE U LJUDSKIM STANICAMA NEUROOBLASTOMA
2016. urn:nbn:hr:193:795541
Manestar, Stela
University of Rijeka
Faculty of Biotechnology and Drug Development

Cite this document

Manestar, S. (2016). PROBIR MODULATORA GAMA SEKRETAZE U LJUDSKIM STANICAMA NEUROOBLASTOMA (Master's thesis). Rijeka: University of Rijeka. Retrieved from https://urn.nsk.hr/urn:nbn:hr:193:795541

Manestar, Stela. "PROBIR MODULATORA GAMA SEKRETAZE U LJUDSKIM STANICAMA NEUROOBLASTOMA." Master's thesis, University of Rijeka, 2016. https://urn.nsk.hr/urn:nbn:hr:193:795541

Manestar, Stela. "PROBIR MODULATORA GAMA SEKRETAZE U LJUDSKIM STANICAMA NEUROOBLASTOMA." Master's thesis, University of Rijeka, 2016. https://urn.nsk.hr/urn:nbn:hr:193:795541

Manestar, S. (2016). 'PROBIR MODULATORA GAMA SEKRETAZE U LJUDSKIM STANICAMA NEUROOBLASTOMA', Master's thesis, University of Rijeka, accessed 18 November 2024, https://urn.nsk.hr/urn:nbn:hr:193:795541

Manestar S. PROBIR MODULATORA GAMA SEKRETAZE U LJUDSKIM STANICAMA NEUROOBLASTOMA [Master's thesis]. Rijeka: University of Rijeka; 2016 [cited 2024 November 18] Available at: https://urn.nsk.hr/urn:nbn:hr:193:795541

S. Manestar, "PROBIR MODULATORA GAMA SEKRETAZE U LJUDSKIM STANICAMA NEUROOBLASTOMA", Master's thesis, University of Rijeka, Rijeka, 2016. Available at: https://urn.nsk.hr/urn:nbn:hr:193:795541

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Metadata
TitlePROBIR MODULATORA GAMA SEKRETAZE U LJUDSKIM STANICAMA NEUROOBLASTOMA
Title (english)SECRETASE MODULATORS IN HUMAN NEUROBLASTOMA CELLS
AuthorStela Manestar
MentorŽeljko Svedružić (mentor)
Committee memberŽeljko Svedružić (predsjednik povjerenstva)
Committee memberJasminka Giacometti (član povjerenstva)
Committee memberKarlo Wittine (član povjerenstva)
GranterUniversity of Rijeka
(Faculty of Biotechnology and Drug Development)
Rijeka
Defense date and country2016-10-27, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Značaj: Alzheimerova bolest je neizlječivi neurodegenerativni poremećaj karakteriziran pojavom amiloidnih plakova u moždanom tkivu. Patofiziologija nastanka amiloidnih plakova povezana je sa poremećajem u metabolizmu APP kojeg katalizira γ-sekretaza. Rezultati: U radu su optimizirani različiti postupci razbijanja stanica na dva tipa ljudskih stanica neuroblastoma. Postupak sa deterdžentom Triton X-100 pokazao se najučinkovitijim. Testirani su dva komercijalna ELISA kita za detekciju Aβ 1-40 proteina te je utvrđeno da je IBL ELISA kit učinkovitiji i cjenovno prihvatljiviji. Na kraju je ispitano inhibicijsko djelovanje 4 potencijalna modulatora γ-sekretaze farmaceutske kuće Jiva Pharma Inc. u dvije različite koncentracije na ljudskim stanicama neuroblastoma. Inhibicijsko djelovanje na γ-sekretazu pokazao je JIVA 028 u koncentraciji 1000 μM, a JIVA 004 i 027 su u istoj koncentraciji bili smrtonosni za stanice. JIVA 026 pojačao je aktivnost γ-sekretaze. Zaključak: U radu je utvrđeno da JIVA 028 pri koncentraciji 1000 μM može inhibirati γ-sekretazu, te se JIVA 028 može dalje istraživati kao potencijalni lijek za Alzheimerovu bolest.
Abstract (english)
Significance: Alzheimer's disease is a terminal neurodegenerative illness characterized by appearance of amyloid plaques in brain tissue. Pathology of amyloid plaques is linked with disorder in APP metabolism whitch is catalyzed by gamma secretase. Results: In this study several procedures for cell lysis were optimized on two types of human neuroblastoma cells. It was found that the best methods is cell lysis by Triton X-100 detergent. Second, two different commercial ELISA kits for measuring Aβ 1-40 were tested, and results suggest that IBL ELISA kit can satisfy our needs in terms of efficiency and price. Third, 4 compounds as modulators of gamma secretase from Jiva Pharma company were tested. Results show that JIVA 028 can inhibit gamma secretase in concentration 1000 μM. JIVA 004 and 027 are letal to cells at concetration 1000 μM. JIVA 026 activates gamma secretase in tested concentrations. Conclusions: JIVA 028 can inhibit gamma secretase at concentration 1000 μM, and as such can be further analyzed as a potential drug for Alzheimer's disease.
Keywords
Languagecroatian
URN:NBNurn:nbn:hr:193:795541
Study programmeTitle: Drug research and development
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra istraživanja i razvoja lijekova (magistar/magistra istraživanja i razvoja lijekova)
Type of resourceText
File originBorn digital
Access conditionsClosed access
Terms of useLicence In copyright icon
Created on2016-11-16 11:42:52