prikaz prve stranice dokumenta Egerolizinski proteini u vodenim ekstraktima izabranih askomiceta i bazidiomiceta
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master's thesis
Egerolizinski proteini u vodenim ekstraktima izabranih askomiceta i bazidiomiceta
2018. urn:nbn:hr:193:744433
Tomić, Kristina
University of Rijeka
Faculty of Biotechnology and Drug Development

Cite this document

Tomić, K. (2018). Egerolizinski proteini u vodenim ekstraktima izabranih askomiceta i bazidiomiceta (Master's thesis). Rijeka: University of Rijeka. Retrieved from https://urn.nsk.hr/urn:nbn:hr:193:744433

Tomić, Kristina. "Egerolizinski proteini u vodenim ekstraktima izabranih askomiceta i bazidiomiceta." Master's thesis, University of Rijeka, 2018. https://urn.nsk.hr/urn:nbn:hr:193:744433

Tomić, Kristina. "Egerolizinski proteini u vodenim ekstraktima izabranih askomiceta i bazidiomiceta." Master's thesis, University of Rijeka, 2018. https://urn.nsk.hr/urn:nbn:hr:193:744433

Tomić, K. (2018). 'Egerolizinski proteini u vodenim ekstraktima izabranih askomiceta i bazidiomiceta', Master's thesis, University of Rijeka, accessed 19 November 2024, https://urn.nsk.hr/urn:nbn:hr:193:744433

Tomić K. Egerolizinski proteini u vodenim ekstraktima izabranih askomiceta i bazidiomiceta [Master's thesis]. Rijeka: University of Rijeka; 2018 [cited 2024 November 19] Available at: https://urn.nsk.hr/urn:nbn:hr:193:744433

K. Tomić, "Egerolizinski proteini u vodenim ekstraktima izabranih askomiceta i bazidiomiceta", Master's thesis, University of Rijeka, Rijeka, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:193:744433

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Metadata
TitleEgerolizinski proteini u vodenim ekstraktima izabranih askomiceta i bazidiomiceta
Title (english)Aegerolysin proteins in water extracts of selected ascomycetes and basidiomycetes
AuthorKristina Tomić
MentorKristina Sepčić (mentor)
MentorAdrijana Leonardi (mentor)
Committee memberSandra Kraljević Pavelić (predsjednik povjerenstva)
Committee memberAnita Jemec (član povjerenstva)
Committee memberAdrijana Leonardi (član povjerenstva)
Committee memberKristina Sepčić (član povjerenstva)
GranterUniversity of Rijeka
(Faculty of Biotechnology and Drug Development)
Rijeka
Defense date and country2018-01-18, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Egerolizini su proteini koji su dosada otkriveni u gljivama, biljkama, praživotinjama, bakterijama, čak i virusima. Prvi izolirani egerolizin je Asp-hemolizin iz plijesni Aspergillus fumigatus. Njihova točna biološka uloga još nije poznata, ali je utvrđeno da ovi proteini, molekulske mase 15-20 kDa, sami ili u kombinaciji s drugim proteinima prave pore u staničnim membranama i umjetnim lipidnim membranama (liposomima). Cilj diplomskog rada je dokazati prisutnost egerolizinskih proteina u višim gljivama (bazidiomicetama) za koje još ne postoje podaci o prisutnosti gena za proteine iz porodice egerolizina, kao i u nižim gljivama (askomicetama) koje u svojim genomima posjeduju gene za egerolizine. Za otkrivanje prisutnosti egerolizina iskoristili smo njihovu sposobnost da se specifično vežu na liposome koji sadrže sfingolipide ceramid fosfoetanolamin ili sfingomijelin u kombinaciji s kolesterolom. Rezultati su pokazali da jedna gljiva posjeduje proteine egerolizine, a nema hemolitičku aktivnost niti sposobnost permeablizacije membrane.
Abstract (english)
Aegerolysins are proteins discovered in fungi, plants, protozoa, bacteria, and even viruses. First isolated aegerolysin was Asp-hemolysin from mould Aspergillus fumigatus. The exact biological role of aegerolysins is not revealed yet, but it was confirmed that these 15-20kDa proteins, alone or accompanied with other proteins induce pores in natural and artificial cell membranes. The main goal of this thesis is to prove the presence of aegerolysins in higher fungi (Basidiomycota) for which there is no available data about the presence of genes coding for aegerolysins proteins, and in the lower fungi (Ascomycota) that harbour aegerolysin-coding genes in their genomes. To discover new aegerolysins, we used their ability to specifically bind to liposomes consisting of ceramide phosphoethanolamine or sphingomyelin in combination with cholesterol. Results showed that only one fungus has aegerolysins proteins, but doesn’t have hemolytical activity nor membrane permeability ability.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:193:744433
Study programmeTitle: Drug research and development
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra istraživanja i razvoja lijekova (magistar/magistra istraživanja i razvoja lijekova)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2018-03-07 08:09:48