prikaz prve stranice dokumenta Analiza interakcija proteinskih biomarkera matičnih stanica raka
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master's thesis
Analiza interakcija proteinskih biomarkera matičnih stanica raka
2017. urn:nbn:hr:193:205245
Kuzelj, Dolores
University of Rijeka
Faculty of Biotechnology and Drug Development

Cite this document

Kuzelj, D. (2017). Analiza interakcija proteinskih biomarkera matičnih stanica raka (Master's thesis). Rijeka: University of Rijeka. Retrieved from https://urn.nsk.hr/urn:nbn:hr:193:205245

Kuzelj, Dolores. "Analiza interakcija proteinskih biomarkera matičnih stanica raka." Master's thesis, University of Rijeka, 2017. https://urn.nsk.hr/urn:nbn:hr:193:205245

Kuzelj, Dolores. "Analiza interakcija proteinskih biomarkera matičnih stanica raka." Master's thesis, University of Rijeka, 2017. https://urn.nsk.hr/urn:nbn:hr:193:205245

Kuzelj, D. (2017). 'Analiza interakcija proteinskih biomarkera matičnih stanica raka', Master's thesis, University of Rijeka, accessed 19 January 2025, https://urn.nsk.hr/urn:nbn:hr:193:205245

Kuzelj D. Analiza interakcija proteinskih biomarkera matičnih stanica raka [Master's thesis]. Rijeka: University of Rijeka; 2017 [cited 2025 January 19] Available at: https://urn.nsk.hr/urn:nbn:hr:193:205245

D. Kuzelj, "Analiza interakcija proteinskih biomarkera matičnih stanica raka", Master's thesis, University of Rijeka, Rijeka, 2017. Available at: https://urn.nsk.hr/urn:nbn:hr:193:205245

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Metadata
TitleAnaliza interakcija proteinskih biomarkera matičnih stanica raka
Title (english)Analysis of the signatures of cancer stem cells in malignant tumours using protein interactomes
AuthorDolores Kuzelj
MentorKrešimir Pavelić (mentor)
Committee memberKrešimir Pavelić (predsjednik povjerenstva)
Committee memberKarlo Wittine (član povjerenstva)
Committee memberIvana Ratkaj (član povjerenstva)
Committee memberSandra Kraljević Pavelić (član povjerenstva)
GranterUniversity of Rijeka
(Faculty of Biotechnology and Drug Development)
Rijeka
Defense date and country2017-10-27, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Cilja rada bio je istražiti biomarkere matičnih stanica raka na temelju literaturnih podataka te izraditi interaktome identificiranih proteinskih biomarkera za analizu interakcija. Također, u eksperimentalnom dijelu, cilj je bio provjeriti rezultate analize interakcija na mezenhimalnim matičnim stanicama (MSC) tretiranim odabranim biomarkerom faktorom rasta živaca (engl. nerve growth factor, NGF). Za izradu interaktoma i analizu proteinskih interakcija koristila se platforma STRING. Rezultati bioinformatičke analize pokazali su kako postoji određeni broj zajedničkih biomarkera između tumorskih matičnih stanica i mezenhimalnih matičnih stanica. Također je pokazano kako niz potencijalnih biomarkera može biti predmet daljnjih analiza i istraživanja u svrhu osmišljavanja novih načina liječenja protiv zloćudnih tumora. Kod mezenhimalnih matičnih stanica naime, NGF je smanjio ekspresiju CD44 i vimentina (VIM). Na temelju analize dobivenih interaktoma može se zaključiti kako je potrebno provesti dodatna istraživanja proteina, osobito NGF koji pokazuju pojačanu ekspresiju kod tumora, ali čija uloga u patogenezi tumora nije u potpunosti jasna.
Abstract (english)
The main purpose of this master thesis was to investigate cancer stem cells biomarkers based on the literature data and to create interactomes for analysis of protein interactions. For interactomes construction and consequent protein interactions analysis, the platform STRING was used. Within the experimental part, the results of interaction analyses were evaluated on mesenchymal stem cells treated with a chosen biomarker, namely nerve growth factor, NGF. Bioinformatics analysis showed a number of shared biomarkers between different cancer stem cells and mesenchymal stem cells. Moreover, several identified biomarkers might be subjected to further research with the aim to evaluate and design novel therapeutic approaches for cancer treatment. Indeed, we showed that in mesenchymal stem cells, NGF lowers expression of CD44 and vimentin. Still it would be necessary to perform additional analyses and research of identified biomarkers, especially NGF that might be over-expressed in different tumours but their role in tumour pathogenesis is still not completely understood.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:193:205245
Study programmeTitle: Biotechnology in medicine
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra biotehnologije u medicini (magistar/magistra biotehnologije u medicini)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2018-01-15 10:29:01