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Diseases linked to the bacterium Porphyromonas gingivalis
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Lucia Fabijančić Porphyromonas gingivalis is a Gram-negative bacterium that is classified as an oral pathogen and, together with the bacteria Treponema denticola and Tannerella forsythia, forms a complex that promotes periodontal diseases such as periodontitis and gingivitis. The main virulence factors of P. gingivalis are cysteine proteinases, gingipains, fimbriae, capsule, lipopolysaccharide layer, and outer membrane vesicles. Some of these virulent factors promote the recruitment of the innate and...
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Dizajn inhibitora ubikvitin tioesteraze OTUB1 pomoću superračunala
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Paulina Grizelj Značaj: Ubikvitinacija je modifikacija u kojoj se na protein kovalentno veže ubikvitin (Ub). Deubikvitinacija je reverzibilni proces ubikvitinaciji, modifikacija u kojoj se uklanja ubikvitin sa proteina. Ravnoteža tih dvaju procesa održava razinu i aktivnost proteina u stanici, a njihova disfunkcija dovodi do nastanka različitih patoloških stanja. Deubikvitinaciju u stanici provode deubikvitinirajući enzimi, kao što je protein ubikvitin tioesteraza OTUB1. Ubikvitin tioesteraza...
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Dizajn linearnih i cikličkih katalitičkih peptida
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Sabino Sepčić Pozadina: Enzimi su proteini koji ubrzavaju kemijske reakcije stvaranjem povoljnih uvjeta za pretvorbu supstrata u produkte. Enzimi su velike makromolekule građene od aminokiselina povezanih peptidnim vezama. Peptidi, koji su također sastavljeni od aminokiselina, su manji i jednostavniji od proteina i mogu imati katalitička svojstva.
Cilj: Glavna svrha ovoga rada je istraživanje promjena svojstva katalize i samoorganizacije kod peptida koje proizlaze iz izmjena aminokiselina.
Metode:...
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Djelovanje tripiridilporfirina na stanice melanoma
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Laura Penić-Ivanko Melanom je maligni tumor melanocita te je zbog svoje agresivnosti i metastatske prirode ujedno i najsmrtonosniji oblik raka kože. Liječenje melanoma uključuje kirurški zahvat, kemoterapiju, radioterapiju, biološku terapiju te lijekove koji specifično ciljaju mutacije povezane s melanomom. Nedostatak ovakvih oblika liječenja je nemogućnost nadilaženja rezistencije melanoma te su iz tog razloga današnja istraživanja usmjerena prema korištenju fotodinamičke terapije kao sigurne i...
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Dopamine and its interaction with DISC1 in animal models
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Ivona Strehovec DISC1 is a scaffold protein described as a risk factor for major mental illnesses, including schizophrenia. It was discovered in Scottish family 23 years ago. The gene was identified at the breakpoint on chromosome 1 of the balanced translocation (1;11) (q42.1; q14.3). In the last few decades there has also been an accent on the role of dopamine in pathophysiology of mental illnesses, specifically the dopamine receptors. Since then, different hypotheses have surfaced about its involvement in...
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Drug-induced and genetic mice models of mania in bipolar disorder
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Matea Kršanac Bipolar disorder is a psychiatric disorder that greatly affects a patient’s day-to-day functioning and is characterised by recurrent manic and depressive episodes. Current mania treatments do not show efficacy for all patients, increasing the need for targeted therapeutic development. The drug development process includes its assessment using applicable animal models, which are sparse for mania in bipolar disorder. An ideal mouse model of mania should fulfil the three validity criteria, as...
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EHD3 aggregation depends on more than one critical region
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Tina Fartek Schizophrenia is a debilitating chronic mental illness that affects people’s
ability to perform daily activities. Schizophrenia is treated with antipsychotics
and psychotherapy which only target the symptoms, however there is little
known about its molecular background. There are several biological theories
of schizophrenia, but the most influential is the dopamine hypothesis, which
states that there is an imbalance in dopamine transmission. Other theories
suggest...
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Editiranje genoma čovjeka: od metodologije do etičkog aspekta
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Kristina Manzoni Pojam editiranje gena odnosi se na izmjenu željenog nukleotidnog slijeda DNA molekule. Otkriće novih, revolucionarnih metoda genetičkog editiranja, od kojih su trenutno najpopularnije i najkorištenije nukleaze cinkovih prstiju (engl. zinc finger nucleases, ZFN), efektorske nukleaze nalik aktivatoru transkripcije (engl. transcription activator-like effector nucleases, TALEN) te niz međusobno razdvojenih kratkih, klasteriranih, palindromskih ponavljanja povezanih s proteinom 9 (engl....
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Effect of disrupting the mRNA turnover on the genome-wide RNA
3'-end structure
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Borna Jurković Uridylation is a common widespread 3'-end modification in eukaryotes;
however, its impact on mRNA fate is not clear. The mRNA decay mechanism
in S. pombe is quite similar to that in humans, where the first step usually
involves uridylation of the 3´-end of mRNA by terminal uridyltransferases
(TUTases). The aim of this project was to investigate the effects on mRNA
decay and mRNA tails when gene expression levels of the factors involved
in mRNA decay are manipulated. We decided...
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Effect of mating and isolation on preferential administration of methamphetamine in D. melanogaster
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Valentina Dukić Many molecular mechanisms are involved in the reward system of the brain. Natural rewards include food, mating and socialization. Artificial rewards include psychostimulative substances, such as methamphetamine (METH). Dopaminergic reward system of fruit fly in Drosophila melanogaster can be stimulated by both natural and artificial rewards. Reward system in D. melanogaster is mainly regulated by signal molecules dopamine (DA) and neuropeptide F (NPF) signalling. Positive social experiences...
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Egerolizinski proteini u vodenim ekstraktima izabranih askomiceta i bazidiomiceta
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Kristina Tomić Egerolizini su proteini koji su dosada otkriveni u gljivama, biljkama, praživotinjama, bakterijama, čak i virusima. Prvi izolirani egerolizin je Asp-hemolizin iz plijesni Aspergillus fumigatus. Njihova točna biološka uloga još nije poznata, ali je utvrđeno da ovi proteini, molekulske mase 15-20 kDa, sami ili u kombinaciji s drugim proteinima prave pore u staničnim membranama i umjetnim lipidnim membranama (liposomima).
Cilj diplomskog rada je dokazati prisutnost egerolizinskih...
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Egerolizinski proteini u vodenim ekstraktima izabranih bazidiomiceta
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Samanta Tonković Egerolizini su obitelj visoko homolognih proteina koja obuhvaća proteine
molekulske mase 15 – 20 kDa. Prvi otkriveni egerolizin je Asp-hemolizin
izoliran iz plijesni Aspergillus fumigatus. Do sada su otkriveni u gljivama,
biljkama, praživotinjama i nekim patogenim bakterijama. Iako još njihova
glavna uloga nije u potpunosti razjašnjena, poznato je kako neki egerolizini
imaju anti-tumorska, antiproliferativna, antibakterijska i hemolitička
svojstva. Također, utvrđeno je da ovi...
