prikaz prve stranice dokumenta Growth phenotype of Saccharomyces cerevisiae taz1Δ: Impact of carbon source and fatty acid supplementation
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master's thesis
Growth phenotype of Saccharomyces cerevisiae taz1Δ: Impact of carbon source and fatty acid supplementation
2024. urn:nbn:hr:193:957839
Sabolek, Emily
University of Rijeka
Faculty of Biotechnology and Drug Development

Cite this document

Sabolek, E. (2024). Growth phenotype of Saccharomyces cerevisiae taz1Δ: Impact of carbon source and fatty acid supplementation (Master's thesis). Rijeka: University of Rijeka. Retrieved from https://urn.nsk.hr/urn:nbn:hr:193:957839

Sabolek, Emily. "Growth phenotype of Saccharomyces cerevisiae taz1Δ: Impact of carbon source and fatty acid supplementation." Master's thesis, University of Rijeka, 2024. https://urn.nsk.hr/urn:nbn:hr:193:957839

Sabolek, Emily. "Growth phenotype of Saccharomyces cerevisiae taz1Δ: Impact of carbon source and fatty acid supplementation." Master's thesis, University of Rijeka, 2024. https://urn.nsk.hr/urn:nbn:hr:193:957839

Sabolek, E. (2024). 'Growth phenotype of Saccharomyces cerevisiae taz1Δ: Impact of carbon source and fatty acid supplementation', Master's thesis, University of Rijeka, accessed 16 June 2024, https://urn.nsk.hr/urn:nbn:hr:193:957839

Sabolek E. Growth phenotype of Saccharomyces cerevisiae taz1Δ: Impact of carbon source and fatty acid supplementation [Master's thesis]. Rijeka: University of Rijeka; 2024 [cited 2024 June 16] Available at: https://urn.nsk.hr/urn:nbn:hr:193:957839

E. Sabolek, "Growth phenotype of Saccharomyces cerevisiae taz1Δ: Impact of carbon source and fatty acid supplementation", Master's thesis, University of Rijeka, Rijeka, 2024. Available at: https://urn.nsk.hr/urn:nbn:hr:193:957839

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Metadata
TitleGrowth phenotype of Saccharomyces cerevisiae taz1Δ: Impact of carbon source and fatty acid supplementation
Title (croatian)Fenotip rasta Saccharomyces cerevisiae taz1Δ: Utjecaj izvora ugljika i suplementacije masnim kiselinama
AuthorEmily Sabolek
MentorChristian Andrew Reynolds (mentor)
Committee memberJasminka Giacometti (predsjednik povjerenstva)
Committee memberStribor Marković (član povjerenstva)
GranterUniversity of Rijeka
(Faculty of Biotechnology and Drug Development)
Rijeka
Defense date and country2024-02-22, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Barth syndrome (BTHS) is an X-linked mitochondrial disorder caused by mutations in the TAZ gene. This gene encodes tafazzin, an enzyme critical for cardiolipin (CL) remodeling, essential for normal mitochondrial structure and function. The absence of CL remodeling is the key pathological mechanism responsible for BTHS. This research employs Saccharomyces cerevisiae as a model organism to understand how different carbon sources impact cellular processes, particularly focusing on non-fermentable sources like pyruvate. The main objectives include characterizing growth defects in tafazzin-deficient cells on various non-fermentable carbon sources and determining the effect of fatty acid (FA) supplementation on cell growth. Growth defects in taz1Δ and cld1Δtaz1Δ mutants on pyruvate indicate the significance of specific carbon sources in cellular processes. FA supplementation partially rescues these defects, suggesting potential connections between FA metabolism, proper mitochondrial function, and pyruvate utilization.
Abstract (croatian)
Barthov sindrom (BTHS) je mitohondrijski poremećaj vezan uz Xkromosom uzrokovan mutacijom u TAZ genu. Ovaj gen kodira tafazzin, enzim ključan za remodeliranje kardiolipina (CL), neophodnog za normalnu strukturu i funkciju mitohondrija. Nedostatak remodeliranja CL ključni je patološki mehanizam odgovoran za BTHS. U ovom se istraživanju koristi Saccharomyces cerevisiae kao modelni organizam za razumijevanje kako različiti izvori ugljika utječu na stanične procese, posebno se fokusirajući na izvore ugljika poput piruvata. Glavni ciljevi istraživanja uključuju karakterizaciju poremećaja rasta stanica kvasca s nedostatkom tafazzina na različitim izvorima ugljika, te određivanje učinka suplementacije masnim kiselinama (MK) na rast stanica. Poremećaji rasta u taz1Δ i cld1Δtaz1Δ mutantima na hranjivim podlogama s piruvatom ukazuju na važnost određenih izvora ugljika u staničnim procesima. Suplementacija MK djelomično spašava ove poremećaje rasta, sugerirajući potencijalne veze između metabolizma MK, pravilne funkcije mitohondrija i iskorištavanja piruvata.
Keywords
Keywords (croatian)
Languageenglish
URN:NBNurn:nbn:hr:193:957839
Study programmeTitle: Biotechnology in medicine
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra biotehnologije u medicini (magistar/magistra biotehnologije u medicini)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to authenticated users
Terms of useLicence In copyright icon
Created on2024-02-21 12:30:06