prikaz prve stranice dokumenta Label-Free Long-Term Methods for Live Cell Imaging of Neurons: New Opportunities
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Scientific paper - Review paper
Label-Free Long-Term Methods for Live Cell Imaging of Neurons: New Opportunities
Biosensors, 13 (2023), 3; 1-15. https://doi.org/10.3390/bios13030404
Baričević, Zrinko; Ayar, Zahra; Leitao, Samuel M.; Mladinic, Miranda; Fantner, Georg E.; Ban, Jelena

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Baričević, Z., Ayar, Z., Leitao, S. M., Mladinic, M., Fantner, G. E. & Ban, J. (2023). Label-Free Long-Term Methods for Live Cell Imaging of Neurons: New Opportunities. Biosensors, 13. (3), 1-15. doi: 10.3390/bios13030404

Baričević, Zrinko, et al. "Label-Free Long-Term Methods for Live Cell Imaging of Neurons: New Opportunities." Biosensors, vol. 13, no. 3, 2023, pp. 1-15. https://doi.org/10.3390/bios13030404

Baričević, Zrinko, Zahra Ayar, Samuel M. Leitao, Miranda Mladinic, Georg E. Fantner and Jelena Ban. "Label-Free Long-Term Methods for Live Cell Imaging of Neurons: New Opportunities." Biosensors 13, no. 3 (2023): 1-15. https://doi.org/10.3390/bios13030404

Baričević, Z., et al. (2023) 'Label-Free Long-Term Methods for Live Cell Imaging of Neurons: New Opportunities', Biosensors, 13(3), pp. 1-15. doi: 10.3390/bios13030404

Baričević Z, Ayar Z, Leitao SM, Mladinic M, Fantner GE, Ban J. Label-Free Long-Term Methods for Live Cell Imaging of Neurons: New Opportunities. Biosensors [Internet]. 2023 March 20 [cited 2024 December 04];13(3):1-15. doi: 10.3390/bios13030404

Z. Baričević, Z. Ayar, S. M. Leitao, M. Mladinic, G. E. Fantner and J. Ban, "Label-Free Long-Term Methods for Live Cell Imaging of Neurons: New Opportunities", Biosensors, vol. 13, no. 3, pp. 1-15, March 2023. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:193:469547. [Accessed: 04 December 2024]

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Metadata
Title (english)Label-Free Long-Term Methods for Live Cell Imaging of Neurons: New Opportunities
AuthorZrinko Baričević
AuthorZahra Ayar
AuthorSamuel M. Leitao
AuthorMiranda Mladinic
AuthorGeorg E. Fantner
AuthorJelena Ban
Author's institutionUniversity of Rijeka
(Faculty of Biotechnology and Drug Development)
Scientific / art field,
discipline and subdiscipline		INTERDISCIPLINARY AREAS OF KNOWLEDGE
Biotechnology in Biomedicine (natural science, biomedicine and healthcare, bioethics area
Abstract (english)
Time-lapse light microscopy combined with in vitro neuronal cultures has provided a significant contribution to the field of Developmental Neuroscience. The establishment of the neuronal polarity, i.e., formation of axons and dendrites, key structures responsible for inter-neuronal signaling, was described in 1988 by Dotti, Sullivan and Banker in a milestone paper that continues to be cited 30 years later. In the following decades, numerous fluorescently labeled tags and dyes were developed for live cell imaging, providing tremendous advancements in terms of resolution, acquisition speed and the ability to track specific cell structures. However, long-term recordings with fluorescence-based approaches remain challenging because of light-induced phototoxicity and/or interference of tags with cell physiology (e.g., perturbed cytoskeletal dynamics) resulting in compromised cell viability leading to cell death. Therefore, a label-free approach remains the most desirable method in long-term imaging of living neurons. In this paper we will focus on label-free high-resolution methods that can be successfully used over a prolonged period. We propose novel tools such as scanning ion conductance microscopy (SICM) or digital holography microscopy (DHM) that could provide new insights into live cell dynamics during neuronal development and regeneration after injury.
Keywords (english)
Languageenglish
Publication typeScientific paper - Review paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleBiosensors
Numberingvol. 13, no. 3, pp. 1-15
p-ISSN2079-6374
DOIhttps://doi.org/10.3390/bios13030404
URN:NBNurn:nbn:hr:193:469547
Publication2023-03-20
ProjectNumber: IP-2016-06-7060
Title (english): Exploring the borderland between neurodegeneration and neuroregeneration: identification of key molecules with proteomics and functional assays in the mammalian spinal cord
Acronym: DefineREgenAgeMode
Leader: M.Mladinić
Jurisdiction: Croatia
ProjectNumber: uniri-biomed-18-258-6427
Title (croatian): Otkrivanje ključnih molekula koje kontroliraju stanične mehanizme neuroprotekcije i neuroregeneracije posredovane proteinima toplinskog stresa nakon in vitro ozljede leđne moždine oposuma
Leader: M.Mladinić
Jurisdiction: Croatia
ProjectNumber: uniriprirod- 18-290-1463
Title (croatian): Identifikacija i kvantifikacija neuralnih matičnih stanica novom metodom optimizirane homogenizacije
Leader: J.Ban
Jurisdiction: Croatia
Document URLhttps://www.mdpi.com/2079-6374/13/3/404
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution 4.0 International (CC BY 4.0) icon
Created on2023-11-15 13:22:25