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undergraduate thesis
ADAR1 onemogućuje aktivaciju protein kinaze R kod replikacije herpes simpleks virusa 1
2023. urn:nbn:hr:193:631053
Čunko, Marina
University of Rijeka
Faculty of Biotechnology and Drug Development

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Čunko, M. (2023). ADAR1 onemogućuje aktivaciju protein kinaze R kod replikacije herpes simpleks virusa 1 (Undergraduate thesis). Rijeka: University of Rijeka. Retrieved from https://urn.nsk.hr/urn:nbn:hr:193:631053

Čunko, Marina. "ADAR1 onemogućuje aktivaciju protein kinaze R kod replikacije herpes simpleks virusa 1." Undergraduate thesis, University of Rijeka, 2023. https://urn.nsk.hr/urn:nbn:hr:193:631053

Čunko, Marina. "ADAR1 onemogućuje aktivaciju protein kinaze R kod replikacije herpes simpleks virusa 1." Undergraduate thesis, University of Rijeka, 2023. https://urn.nsk.hr/urn:nbn:hr:193:631053

Čunko, M. (2023). 'ADAR1 onemogućuje aktivaciju protein kinaze R kod replikacije herpes simpleks virusa 1', Undergraduate thesis, University of Rijeka, accessed 29 December 2024, https://urn.nsk.hr/urn:nbn:hr:193:631053

Čunko M. ADAR1 onemogućuje aktivaciju protein kinaze R kod replikacije herpes simpleks virusa 1 [Undergraduate thesis]. Rijeka: University of Rijeka; 2023 [cited 2024 December 29] Available at: https://urn.nsk.hr/urn:nbn:hr:193:631053

M. Čunko, "ADAR1 onemogućuje aktivaciju protein kinaze R kod replikacije herpes simpleks virusa 1", Undergraduate thesis, University of Rijeka, Rijeka, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:193:631053

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Metadata
TitleADAR1 onemogućuje aktivaciju protein kinaze R kod replikacije herpes simpleks virusa 1
Title (english)ADAR1 prevents protein kinase R activation in herpes simplex virus 1 infection
AuthorMarina Čunko
MentorIgor Jurak (mentor)
Committee memberŽeljka Maglica (predsjednik povjerenstva)
Committee memberMladen Merćep (član povjerenstva)
Committee memberIgor Jurak (član povjerenstva)
GranterUniversity of Rijeka
(Faculty of Biotechnology and Drug Development)
Rijeka
Defense date and country2023-09-21, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Adenozin deaminaza koja djeluje na RNA (ADAR, prema eng. adenosine deaminase acting on RNA) katalizira pretvorbu adenozina u inozin na dvolančanim RNA molekulama. Pokazalo se da ADAR1, član obitelji ADAR proteina, može ciljati i virusne RNA transkripte te utjecati na proces replikacije virusa. Ovisno o virusu, njegova uloga može biti provirusna ili antivirusna. Pro- i antivirusne funkcije ADAR1 enzima dobro su istražene za brojne RNA viruse, ali malo se zna o njihovoj ulozi u replikaciji DNA virusa. U našem istraživanju, koristili smo HEK293 stanice sa stabilnom delecijom ADAR1 gena (ADAR1 KO stanice) kako bi testirali učinak nedostatka ADAR1 u infekciji herpes simpleks virusom tip 1 (HSV-1), široko rasprostranjenom ljudskom DNA virusu. Otkrili smo da je replikacija HSV-1 otežana u stanicama s nedostatkom ADAR1 u usporedbi s kontrolnim stanicama. Dodatno, istražili smo potencijalne mehanizme kojima ADAR1 ostvaruje svoj provirusni efekt. Provjerili smo razine proteina uključenih u signalne puteve urođene imunosti, kao i njihove posttranslacijske modifikacije. Otkrili smo da smanjena replikacija HSV-1 u ADAR1 KO stanicama korelira s pojačanom aktivacijom signalnog puta PKR kinaze, ključne kinaze za aktivaciju urođenih protuvirusnih odgovora domaćina. Zajedno, naši rezultati sugeriraju da ADAR1 sprječava PKR-posredovan prekid translacije proteina te time ispoljava provirusni učinak na infekciju HSV-1.
Abstract (english)
Adenosine deaminase acting on RNA (ADAR) catalyzes the conversion of adenosine to inosine on double-stranded RNA molecules. It has been shown that ADAR1, a member of the ADAR protein family, can target viral RNA transcripts and affect the virus replication process. Depending on the virus, its role can be either proviral or antiviral. The pro- and antiviral functions of ADAR1 have been well studied for numerous RNA viruses, but little is known about their role in DNA virus replication. In our study, we utilized HEK293 cells with a stable deletion of the ADAR1 (ADAR1 KO) to test the effect of ADAR1 deficiency in herpes simplex virus 1 (HSV-1) infection, a widespread human DNA virus. We found that HSV-1 replication is impaired in ADAR1-deficient cells compared to control cells. Additionally, we investigated the potential mechanisms through which ADAR1 exerts its proviral effect. We analyzed the levels of proteins involved in the innate immunity signaling pathways, as well as their post-translational modifications. We found that reduced HSV-1 replication in ADAR1 KO cells correlates with the enhanced activation of the PKR kinase signaling pathway, a key kinase for the activation of host innate antiviral responses. Taken together, our findings suggest that ADAR1 suppresses the PKR-mediated translational shutdown, thus having a proviral effect on HSV-1 infection.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:193:631053
Study programmeTitle: Biotechnology and drug research
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica biotehnologije i istraživanja lijekova (sveučilišni/a prvostupnik/prvostupnica biotehnologije i istraživanja lijekova)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2023-10-08 17:35:06