Title Stabilnost gallij-dopamin nanočestica u različitim vodenim medijima Title (croatian) Stabilnost galij-dopamin nanočestica u različitim vodenim medijima Author Iva Hudoletnjak Mentor Mladenka Malenica (mentor)Mentor Paula Žurga (komentor)Committee member Mladenka Malenica (predsjednik povjerenstva)Committee member Paula Žurga (član povjerenstva)Committee member Toni Todorovski (član povjerenstva)Committee member Dean Marković (član povjerenstva)Committee member Milan Mesić (član povjerenstva)Granter University of Rijeka Defense date and country 2023-09-27, Croatia Scientific / art field, BIOTECHNICAL SCIENCES Abstract Antibiotic resistance is slowly becoming a serious problem in global public health due to antibiotic overuse, and due to a significant rise in multi-drug resistant bacteria. A lot of research has been put into finding antibacterial alternatives. Metal ions and metal-based nanoparticles are currently being studied as an alternative to antibiotics. Among various metals with antibacterial properties, gallium (Ga) ions and their nanoparticles have attracted attention due to their antibacterial properties. However, due to the low bioavailability of gallium ions, and the unique physicochemical properties of nanoparticles, gallium nanoparticles (Ga NPs) might be a better alternative compared to ions. Factors that can influence the antibacterial efficiency of Ga NPs are yet to be researched. In this work, the change in concentration of Ga NPs and their dissolution at different temperatures (20 °C and 37 °C) was measured in various aqueous media: distilled water, phosphate saline buffer (PBS), and bacterial growth medium (Mueller Hinton Broth, MHB). Determination of gallium ions concentration and Ga NPs was performed by inductively coupled plasma mass spectrometry (ICP-MS). Dissolution and ICP-MS ion release profiles of Ga NPs in different aqueous solutions and at different temperatures were observed and compared. Results suggest that the rates of dissolution differ in all of the investigated solutions and that temperature affects dissolution. Ga NPs at lower temperatures are more stable in all three solutions and have fewer statistical deviations. At higher temperatures, dissolutions went over 100 % in PBS and MHB. Even though Ga NPs are very stable in distilled H2O, distilled water has low dissolution rates, which might not be ideal for the antibacterial purpose of Ga NPs. Dissolution of Ga NPs is consistent, slowly increasing over 24 hours, at 20 °C. Around 80 % dissolution is detected in PBS and almost 100 % in MHB after 24 h, at lower temperatures. The effects of aqueous media on Ga NPs dissolution demonstrate that both PBS and MHB enable favorable dissolution, especially at 20 °C.
Abstract (croatian) Otpornost na antibiotike polako postaje ozbiljan problem u svjetskom javnom zdravstvu zbog prekomjerne uporabe antibiotika i značajnog porasta bakterija koje su otporne na više vrsta lijekova. Puno je istraživanja uloženo u pronalaženje antibakterijskih alternativa. Metalni ioni i nanočestice bazirane na metalima trenutno se proučavaju kao alternativa antibioticima. Među raznim metalima s antibakterijskim svojstvima, ioni galija (Ga) i njegove nanočestice privukli su pozornost zbog antibakterijskih svojstava. Međutim, zbog niske bioraspoloživosti iona galija i jedinstvenih fizikalno-kemijskih svojstava nanočestica, nanočestice galija (engl. gallium nanoparticles, Ga NP) mogle bi biti bolja alternativa nego ioni. Čimbenici koji mogu utjecati na antibakterijsku učinkovitost Ga NP tek treba istražiti. U ovom radu, promjena koncentracije Ga NP i njihovo otapanje na različitim temperaturama (20 °C i 37 °C) mjerit će se u različitim vodenim medijima: destilirana voda, fosfatni pufer i medij za rast bakterija (Mueller Hinton Broth, MHB). Određivanje koncentracije galijevih iona i Ga NPs provedeno je pomoću spektrometrije masa uz induktivno spregnutu plazmu (ICP-MS). Promatrani su i uspoređeni profili otapanja nanočestica i otpuštanja Ga iona u različitim vodenim otopinama i na različitim temperaturama. Rezultati pokazuju da se otapanja Ga NP razlikuju u svim ispitivanim otopinama, te da temperatura utječe na otapanje. Ga NP na nižim temperaturama stabilnije su u sve tri otopine i rezultati imaju manja statistička odstupanja. Pri višim temperaturama dobivene su vrijednosti otapanja preko 100 % u PBS i MHB. Iako su Ga NP vrlo stabilne u destiliranoj H2O, destilirana voda ima niske stope otapanja, što možda nije idealno za antibakterijsku svrhu Ga NP. Otapanje Ga NP je dosljedno, polako se povećava tijekom vremenskog raspona od 24 sata, na 20 °C. Oko 80 % otapanja detektirano je u PBS-u i gotovo 100 % u MHB-u nakon 24 sata, na nižoj temperaturi. Učinci vodenih medija na otapanje Ga NP pokazuju da i PBS i MHB omogućuju povoljno otapanje nanočestica, posebno na 20 °C.
Keywords
gallium nanoparticles
dissolution
aqueous media
ICP-MS
temperature
Keywords (croatian)
galijeve nanočestice
otapanje
vodeni mediji
ICP-MS
temperatura.
Language english URN:NBN urn:nbn:hr:193:480148 Study programme Title: Biotechnology in medicine Type of resource Text File origin Born digital Access conditions Embargoed access Terms of use Created on 2023-09-27 14:42:35
