prikaz prve stranice dokumenta Introduction of quantitative PCR for determination of the number of newly synthesized MCMV
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master's thesis
Introduction of quantitative PCR for determination of the number of newly synthesized MCMV
2023. urn:nbn:hr:193:516926
Belužić, Ema
University of Rijeka
Faculty of Biotechnology and Drug Development

Cite this document

Belužić, E. (2023). Introduction of quantitative PCR for determination of the number of newly synthesized MCMV (Master's thesis). Rijeka: University of Rijeka. Retrieved from https://urn.nsk.hr/urn:nbn:hr:193:516926

Belužić, Ema. "Introduction of quantitative PCR for determination of the number of newly synthesized MCMV." Master's thesis, University of Rijeka, 2023. https://urn.nsk.hr/urn:nbn:hr:193:516926

Belužić, Ema. "Introduction of quantitative PCR for determination of the number of newly synthesized MCMV." Master's thesis, University of Rijeka, 2023. https://urn.nsk.hr/urn:nbn:hr:193:516926

Belužić, E. (2023). 'Introduction of quantitative PCR for determination of the number of newly synthesized MCMV', Master's thesis, University of Rijeka, accessed 05 May 2024, https://urn.nsk.hr/urn:nbn:hr:193:516926

Belužić E. Introduction of quantitative PCR for determination of the number of newly synthesized MCMV [Master's thesis]. Rijeka: University of Rijeka; 2023 [cited 2024 May 05] Available at: https://urn.nsk.hr/urn:nbn:hr:193:516926

E. Belužić, "Introduction of quantitative PCR for determination of the number of newly synthesized MCMV", Master's thesis, University of Rijeka, Rijeka, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:193:516926

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Metadata
TitleIntroduction of quantitative PCR for determination of the number of newly synthesized MCMV
Title (croatian)Uvođenje kvantitativnog PCRa za određivanja broja novosintetiziranih MCMV
AuthorEma Belužić
MentorLjerka Karleuša (mentor)
Committee memberIgor Jurak (predsjednik povjerenstva)
Committee memberKatarina Kapuralin (član povjerenstva)
GranterUniversity of Rijeka
(Faculty of Biotechnology and Drug Development)
Rijeka
Defense date and country2023-09-26, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
In the research on virology, accurate and sensitive methods for detecting viral pathogens are essential. This study presents comparative analysis of two commonly used techniques, quantitative PCR (qPCR) and plaque assays, for the detection of murine cytomegalovirus (MCMV). Cytomegalovirus (CMV), a member of the Herpesviridae family, causes medical complications in immunocompromised individuals including congenital defects, post-transplant graft rejections and cardiovascular diseases. CMV infection is complex due to its ability to establish latency in its host and capacity to establish lifelong infections. Understanding its dynamics, especially in immunocompromised individuals, is important. The paper presented shows the efficacy of qPCR as a tool for MCMV quantification, presenting its capability to detect viral DNA even in latent or non-active states. Our findings show a considerable growth in sensitivity when using qPCR compared to the conventional plaque assay. qPCR yielded higher viral particles concentrations and demonstrated advantages in terms of precision and quantification accuracy. qPCR sensitivity allows the detection of minimal quantities of MCMV genome, giving us a better understanding of the virus's latent phase and improving our ability to measure viral dynamics. Furthermore, qPCR's ability to provide results with a faster processing time and reduced subjectivity compared to plaque assays presents it as a useful tool for research and clinical applications. The study underlines the potential of qPCR to improve MCMV detection and quantification, showing a better approach in studying the virus, evaluating antiviral treatments, and eventually improving our ability to treat CMV-related diseases.
Abstract (croatian)
U istraživanjima u području virologije, precizne i osjetljive metode za otkrivanje virusnih patogena su esencijalne. Ova studija predstavlja komparativnu analizu dviju često korištenih tehnika, kvantitativnog PCR (qPCR) i testova plakova, za detekciju mišjeg citomegalovirusa (MCMV). Citomegalovirus (CMV), član obitelji Herpesviridae, uzrokuje medicinske komplikacije kod imunokompromitiranih pojedinaca uključujući urođene defekte, odbacivanje presatka nakon transplantacije i kardiovaskularne bolesti. CMV infekcija složena je zbog svoje sposobnosti uspostavljanja latencije u svom domaćinu i sposobnosti uspostavljanja cjeloživotnih infekcija. Razumijevanje njegove dinamike, posebno u imunokompromitiranih osoba, iznimno je važno. Predstavljeni rad pokazuje učinkovitost qPCR-a kao metode za kvantifikaciju MCMV-a, prikazujući njegovu sposobnost detekcije virusne DNA čak i u latentnom ili neaktivnom stanju. Naši rezultati pokazuju značajan porast osjetljivosti pri korištenju qPCR-a u usporedbi s konvencionalnim testovima plakova. qPCR detektirao je veće koncentracije virusnih čestica i pokazao prednosti u pogledu preciznosti i točnosti kvantifikacije. Osjetljivost qPCR-a omogućuje otkrivanje minimalnih količina MCMV genoma, što nam daje bolje razumijevanje latentne faze virusa i poboljšava našu sposobnost mjerenja dinamike virusne infekcije. Nadalje, sposobnost qPCR-a da pruži rezultate s bržim vremenom obrade i smanjenom subjektivnošću u usporedbi s analizama plakova predstavlja ga kao koristan alat za istraživanja i kliničke primjene. Studija naglašava potencijal qPCR-a za poboljšanje detekcije i kvantifikacije MCMV-a, predstavljajući bolji pristup u proučavanju virusa, procjeni antivirusnih tretmana i na kraju poboljšanju naše sposobnosti liječenja bolesti povezanih s CMV-om.
Keywords
Keywords (croatian)
Languageenglish
URN:NBNurn:nbn:hr:193:516926
Study programmeTitle: Biotechnology in medicine
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra biotehnologije u medicini (magistar/magistra biotehnologije u medicini)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2023-09-25 09:05:50