Barth syndrome is a rare recessive genetic disorder. It mostly affects mitochondria-rich organs like the heart and skeletal muscles. However, there is a wide range of symptoms like cardiomyopathy, skeletal myopathy, hypertrophy, fibrosis, increased urinary excretion of 3- methylglutaconic acid, learning disabilities, attention deficit and so on. The disorder is caused by a mutation in the tafazzin (TAZ) gene on the X chromosome. TAZ gene codes for the tafazzin enzyme that participates in a mechanism of cardiolipin (CL) maturation. CL is a phospholipid located in the inner mitochondrial membrane. It provides membrane homeostasis and cell integrity. CL mutations make mitochondrial energy production less effective and shorter cell life. Since the physiology behind CL synthesis is not completely understood patients with Barth syndrome are treated symptomatically. However, with a better understanding of biochemistry and molecular mechanism connected to CL, new treatment methods could be considered. One of them is a high-protein diet. Proteins are macromolecules comprised of long chains of amino acids. Amino acids are building blocks for a variety of compounds. In some research amino acid supplementation improves cell growth. That is why we are interested in what effects supplementation of amino acids has on TAZ-deficient S. cerevisiae cells.