Aluminum is a metal in the 13th group of the periodic table of elements and has no physiological role in the body. It has been linked to a number of diseases such as Alzheimer's, Parkinson's, Down syndrome, autism, etc. Increasing global climate change has led to an increase in the concentration of aluminum in the environment and its entry into the biota. Currently, there is no method to remove it from the environment, or to detoxify living organisms, including humans. The existing therapy for aluminum salt poisoning is desferrioxamine. As it has large number of side effects, in recent years possibility of using alternative ways of detoxification of aluminum in living organisms has been investigated, for example by using clinoptilolite zeolites which are known ion exchangers, adsorbents and orthosilicic acid realising compounds which is also extremely important in the context of aluminum detoxification. Therefore, the physicochemical properties of natural zeolite clinoptilolite materials tribomechanically activated clinoptilolite TMAZ, double tribomechanically activated zeolite PMA and double tribomechanically activated oxygenated zeolite PMAO2 were investigated in this doctorate and synthetic zeolite A was used as a control compound. Physicochemical properties of zeolite materials are important for explaining interactions with ions and biomolecules in biological systems and in this doctorate were performed experiments with Fourier transformed infrared spectroscopy, determination of zeta potential, pH of zeolite suspensions in model solutions of intestine and stomach as well as in water, in vitro binding capacity of heavy metals, determination of particle size by dynamic light scattering and surface analysis of the materials by XPS / EDS. All investigated clinoptilolite materials showed a significant binding capacity of lead cations in model solution of stomach and intestines and arsen, chromium and nickel in the model solution of the intestines. Furthermore, all zeolite clinoptilolite materials showed detoxifying properties on aluminum in vivo in a model of intoxicated rats by subchronic doses of aluminum chloride. The mechanism of action of this property was investigated for PMAO2 analysis of animal liver by DNA microchip method which showed activation of Sfrp1, Cry1, Rassf8 genes and supression of Nr1d2 genes in liver tissue involved in detoxification mechanisms. These results support the use of clinoptilolite zeolite for medical purposes.