prikaz prve stranice dokumenta Computational and experimental therapeutic efficacy analysis of andrographolide phospholipid complex self-assembled nanoparticles against Neuro2a cells
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Scientific paper - Original scientific paper
Computational and experimental therapeutic efficacy analysis of andrographolide phospholipid complex self-assembled nanoparticles against Neuro2a cells
Biochimica et Biophysica Acta (BBA) - General Subjects, 1867 (2022), 2; 130283. https://doi.org/10.1016/j.bbagen.2022.130283
Mourya, Atul; Pingle, Purva; Babu, Chanti Katta; Veerabomma, Harithasree; Sainaga Jyothi, Vaskuri G.S; Novak, Jurica; Pathak, Prateek; Grishina, Maria; Verma, Amita; Kumar, Rahul; Singh, Pankaj Kumar; Khatri, Dharmendra Kumar; Singh, Shashi Bala; Madan, Jitender

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Mourya, A., Pingle, P., Babu, C. K., Veerabomma, H., Sainaga Jyothi, V. G., Novak, J. ... Madan, J. (2022). Computational and experimental therapeutic efficacy analysis of andrographolide phospholipid complex self-assembled nanoparticles against Neuro2a cells. Biochimica et Biophysica Acta (BBA) - General Subjects, 1867. (2). doi: 10.1016/j.bbagen.2022.130283

Mourya, Atul, et al. "Computational and experimental therapeutic efficacy analysis of andrographolide phospholipid complex self-assembled nanoparticles against Neuro2a cells." Biochimica et Biophysica Acta (BBA) - General Subjects, vol. 1867, no. 2, 2022. https://doi.org/10.1016/j.bbagen.2022.130283

Mourya, Atul, Purva Pingle, Chanti Katta Babu, Harithasree Veerabomma, Vaskuri G.S Sainaga Jyothi, Jurica Novak, Prateek Pathak, et al. "Computational and experimental therapeutic efficacy analysis of andrographolide phospholipid complex self-assembled nanoparticles against Neuro2a cells." Biochimica et Biophysica Acta (BBA) - General Subjects 1867, no. 2 (2022). https://doi.org/10.1016/j.bbagen.2022.130283

Mourya, A., et al. (2022) 'Computational and experimental therapeutic efficacy analysis of andrographolide phospholipid complex self-assembled nanoparticles against Neuro2a cells', Biochimica et Biophysica Acta (BBA) - General Subjects, 1867(2). doi: 10.1016/j.bbagen.2022.130283

Mourya A, Pingle P, Babu CK, Veerabomma H, Sainaga Jyothi VG, Novak J, and sur.. Computational and experimental therapeutic efficacy analysis of andrographolide phospholipid complex self-assembled nanoparticles against Neuro2a cells. Biochimica et Biophysica Acta (BBA) - General Subjects [Internet]. 2022 November 19 [cited 2024 May 05];1867(2). doi: 10.1016/j.bbagen.2022.130283

A. Mourya, et al., "Computational and experimental therapeutic efficacy analysis of andrographolide phospholipid complex self-assembled nanoparticles against Neuro2a cells", Biochimica et Biophysica Acta (BBA) - General Subjects, vol. 1867, no. 2, November 2022. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:193:590974. [Accessed: 05 May 2024]

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Title (english)Computational and experimental therapeutic efficacy analysis of andrographolide phospholipid complex self-assembled nanoparticles against Neuro2a cells
AuthorAtul Mourya
AuthorPurva Pingle
AuthorChanti Katta Babu
AuthorHarithasree Veerabomma
AuthorVaskuri G.S Sainaga Jyothi
AuthorJurica Novak
AuthorPrateek Pathak https://orcid.org/0000-0002-6160-9755
AuthorMaria Grishina
AuthorAmita Verma
AuthorRahul Kumar
AuthorPankaj Kumar Singh
AuthorDharmendra Kumar Khatri
AuthorShashi Bala Singh
AuthorJitender Madan
Author's institutionUniversity of Rijeka
(Faculty of Biotechnology and Drug Development)
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Applied Chemistry
Abstract (english)
Background: Neuroblastoma is one of the most common malignancies in childhood, accounts for approximately 7% of all malignancies. Andrographolide (AN) inhibits cancer cells progression via multiple pathways like cell cycle arrest, mitochondrial apoptosis, NF-κβ inhibition, and antiangiogenesis mechanism. Despite multiple advantages, application of AN is very limited due to its low aqueous solubility (6.39 ±0.47 μg/mL), high lipophilicity (log P ~ 2.632 ±0.135), and reduced stability owing to pH sensitive lactone ring. Objectives and results: In present investigation, a molecular complex of AN with soya-L-α-phosphatidyl choline (SPC) was synthesized as ANSPC and characterized by FT-IR and1H NMR spectroscopy. Spectral and molecular simulation techniques confirmed the intermolecular interactions between the 14-OH group of AN and the N+(CH3)3 part of SPC. In addition, molecular dynamics (MD) simulation was used to determine the degree of interaction between various proteins such as TNF-α, caspase-3, and Bcl-2. Later, ANSPC complex was transformed in to self-assembled soft nanoparticles of size 201.8 ±1.48 nm with PDI of 0.092 ± 0.004 and zeta potential of 21.7 ± 0.85 mV. The IC50 of free AN (8.319 μg/mL) and the self-assembled soft ANSPC nano-particles (3.406 μg/mL ~ 1.2 μg of AN) against Neuro2a cells was estimated with significant (P <0.05) difference. Interestingly, the self-assembled soft ANSPC nanoparticles showed better endocytosis compared to free AN in Neuro2a cells. In vitro biological assays confirmed that self-assembled soft ANSPC nanoparticles induces apoptosis in Neuro2a cells by declining the MMP (Δψm) and increasing the ROS generation. Conclusion: Self-assembled soft ANSPC nanoparticles warrant further in-depth antitumor study in xenograft model of neuroblastoma to establish the anticancer potential.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionAccepted version
Journal titleBiochimica et Biophysica Acta (BBA) - General Subjects
Numberingvol. 1867, no. 2, 130283
p-ISSN0304-4165
DOIhttps://doi.org/10.1016/j.bbagen.2022.130283
URN:NBNurn:nbn:hr:193:590974
Online first2022-11-19
Document URLhttps://www.sciencedirect.com/science/article/pii/S030441652200201X
Type of resourceText
Access conditionsOpen access
Embargo expiration date: 2024-01-01
Terms of useLicence Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) icon
Created on2022-11-30 13:21:12