Bacteria of the genus Enterococcus are Gram-positive, anaerobic pathogens that are naturally found in the gastrointestinal tract of humans, and the most common species is Enterococcus faecalis. Although they are considered commensal bacteria, they very often cause urinary infections - infections present in any part of the urinary tract. Urinary tract infections are one of the most common bacterial infections and are one of the most common reasons for visiting a doctor. In addition to urinary infections, enetrococci are often the cause of endocarditis, intra-abdominal infections, neonatal sepsis, meningitis and nosocomial bacteremia. Due to the increased resistance of enterococci to antimicrobial agents, it is advisable to investigate other alternative approaches that would enable efficient therapy and prophylaxis. Therefore, in this study, we were examining the influence of different antimicrobial substances on the growth curve of two strains of E. faecalis. The impact of two antibiotics was tested: nitroxolin, an effective antibiotic in the fight against biofilm infections whose antimicrobial action is based on chelation, and gentamicin, an aminoglycoside antibiotic with a wide range of bactericidal action. The influence of hydroquinone, which is an aromatic, phenolic organic compound created by the breakdown of arbutin in the enterocytes of the intestinal villi, was also tested. The aim of the research was to examine how different antimicrobial substances in different concentrations act both individually and in combinations on the growth curves of E. faecalis strain 36 and strain ATCC29212. Also, on the basis of minimum inhibitory concentration (MIC) and fractional inhibitory concentration (FIC), interactions between combinations of antimicrobial substances were determined. The results showed great potential between different concentrations of the combination of nitroxoline and hydroquinone, so this combination of compounds needs to be further investigated.