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undergraduate thesis
Solid Phase Peptide Synthesis
2022. urn:nbn:hr:193:993065
Moržan, Sara
University of Rijeka
Faculty of Biotechnology and Drug Development

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Moržan, S. (2022). Solid Phase Peptide Synthesis (Undergraduate thesis). Rijeka: University of Rijeka. Retrieved from https://urn.nsk.hr/urn:nbn:hr:193:993065

Moržan, Sara. "Solid Phase Peptide Synthesis." Undergraduate thesis, University of Rijeka, 2022. https://urn.nsk.hr/urn:nbn:hr:193:993065

Moržan, Sara. "Solid Phase Peptide Synthesis." Undergraduate thesis, University of Rijeka, 2022. https://urn.nsk.hr/urn:nbn:hr:193:993065

Moržan, S. (2022). 'Solid Phase Peptide Synthesis', Undergraduate thesis, University of Rijeka, accessed 10 May 2024, https://urn.nsk.hr/urn:nbn:hr:193:993065

Moržan S. Solid Phase Peptide Synthesis [Undergraduate thesis]. Rijeka: University of Rijeka; 2022 [cited 2024 May 10] Available at: https://urn.nsk.hr/urn:nbn:hr:193:993065

S. Moržan, "Solid Phase Peptide Synthesis", Undergraduate thesis, University of Rijeka, Rijeka, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:193:993065

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Metadata
TitleSolid Phase Peptide Synthesis
Title (croatian)Sinteza peptida na čvrstom nosaču
AuthorSara Moržan
MentorDaniela Kalafatović (mentor)
Committee memberNela Malatesti (predsjednik povjerenstva)
Committee memberJelena Ban (član povjerenstva)
GranterUniversity of Rijeka
(Faculty of Biotechnology and Drug Development)
Rijeka
Defense date and country2022-09-08, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Peptides are biomolecules composed of two to fifty amino acids, connected into chains through peptide bonds. They have a wide variety of applications in medicine, biology, chemistry and other biomedical-related fields because of their antimicrobial, antiviral and catalytic activities. Solid phase peptide synthesis (SPPS) is the method used for producing these compounds in the laboratory. It consists of a few basic steps, namely: deprotection, coupling, washing, acetylation, and cleavage. This undergraduate thesis is focused on the Fmoc SPPS synthesis, analysis, and purification of the Ac-HREAKRTRVCYR-Am (Ac=acyl group; Am=amide group) peptide and provides summarized protocols for all used methods. During the synthesis, difficulties regarding the removal of the protecting group and liquid chromatography-mass spectrometry analysis were encountered due to the high hydrophilicity of the peptide. For this reason, the aim was to find different ways to optimize the method for synthesis, characterization, and purification to achieve a peptide of high purity that could undergo different biocatalytic assays in the future.
Abstract (croatian)
Peptidi su biomolekule sastavljene od 2 do 50 aminokiselina povezanih u lanac peptidnim vezama. Imaju široku primjenu u medicini, biologiji, kemiji i ostalim srodnim biomedicinskim područjima zbog antimikrobnih, antivirusnih i katalitičkih sposobnosti. Sinteza peptida na čvrstom nosaču (engl. solid phase peptide synthesis; SPPS) metoda je koja se koristi za proizvodnju ovih spojeva u laboratoriju. Sastoji se od nekoliko osnovnih koraka: uklanjanje zaštitne skupine s aminokiselina, spajanja aminokiselina u lanac (engl. coupling), ispiranja, acetilacije i skidanja peptida s nosača (engl. cleavage). Ovaj završni rad usmjeren je na Fmoc SPPS sintezu, analizu i pročišćavanje peptida Ac-HREAKRTRVCYR-Am (Ac=acilna skupina; Am=amidna skupina) i pruža sažete protokole za sve korištene metode. Tijekom sinteze pojavile su se poteškoće s uklanjanjem zaštitne skupine i analize tekućinskom kromatografijom zbog visoke hidrofilnosti peptida. Iz tog razloga, cilj je bio pronaći različite načine za optimizaciju metoda sinteze, karakterizacije i pročišćavanja kako bi dobili peptid visoke čistoće koji može biti podvrgnut različitim biokatalitičkim testovima u budućnosti
Keywords
Keywords (croatian)
Languageenglish
URN:NBNurn:nbn:hr:193:993065
Study programmeTitle: Biotechnology and drug research
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica biotehnologije i istraživanja lijekova (sveučilišni/a prvostupnik/prvostupnica biotehnologije i istraživanja lijekova)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2022-09-07 06:26:23