prikaz prve stranice dokumenta Development of novel protocols for polyethylene terephthalate and polystyrene isolation from seawater samples and their identification using ATR-FTIR and MALDI-MS
Embargo period 2027-07-27
master's thesis
Development of novel protocols for polyethylene terephthalate and polystyrene isolation from seawater samples and their identification using ATR-FTIR and MALDI-MS
2022. urn:nbn:hr:193:669565
Radović, Erika
University of Rijeka
Faculty of Biotechnology and Drug Development

Cite this document

Radović, E. (2022). Development of novel protocols for polyethylene terephthalate and polystyrene isolation from seawater samples and their identification using ATR-FTIR and MALDI-MS (Master's thesis). Rijeka: University of Rijeka. Retrieved from https://urn.nsk.hr/urn:nbn:hr:193:669565

Radović, Erika. "Development of novel protocols for polyethylene terephthalate and polystyrene isolation from seawater samples and their identification using ATR-FTIR and MALDI-MS." Master's thesis, University of Rijeka, 2022. https://urn.nsk.hr/urn:nbn:hr:193:669565

Radović, Erika. "Development of novel protocols for polyethylene terephthalate and polystyrene isolation from seawater samples and their identification using ATR-FTIR and MALDI-MS." Master's thesis, University of Rijeka, 2022. https://urn.nsk.hr/urn:nbn:hr:193:669565

Radović, E. (2022). 'Development of novel protocols for polyethylene terephthalate and polystyrene isolation from seawater samples and their identification using ATR-FTIR and MALDI-MS', Master's thesis, University of Rijeka, accessed 23 January 2025, https://urn.nsk.hr/urn:nbn:hr:193:669565

Radović E. Development of novel protocols for polyethylene terephthalate and polystyrene isolation from seawater samples and their identification using ATR-FTIR and MALDI-MS [Master's thesis]. Rijeka: University of Rijeka; 2022 [cited 2025 January 23] Available at: https://urn.nsk.hr/urn:nbn:hr:193:669565

E. Radović, "Development of novel protocols for polyethylene terephthalate and polystyrene isolation from seawater samples and their identification using ATR-FTIR and MALDI-MS", Master's thesis, University of Rijeka, Rijeka, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:193:669565

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Metadata
TitleDevelopment of novel protocols for polyethylene terephthalate and polystyrene isolation from seawater samples and their identification using ATR-FTIR and MALDI-MS
Title (croatian)Razvoj novih protokola za izolaciju polietilen tereftalata i polistirena iz morskih uzoraka te njihova identifikacija pomoću ATR-FTIR-a i MALDI-MS-a
AuthorErika Radović
MentorDaniela Kalafatović (mentor)
Committee memberNela Malatesti (predsjednik povjerenstva)
Committee memberLado Kranjčević (član povjerenstva)
Committee memberDaniela Kalafatović (član povjerenstva)
GranterUniversity of Rijeka
(Faculty of Biotechnology and Drug Development)
Rijeka
Defense date and country2022-07-27, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Polymers are macromolecules with repeated structural units. Synthetic polymers have first been introduced in the middle of the 19th century, and since then their production has exponentially increased on yearly basis. As of 2015, more than 6300 Mt of plastic waste has been generated throughout the world, with more than 76% of that waste ending in landfills and oceans. To fully estimate the effect of this pollution on our planet, simple standardized protocols should be set in place for microplastic analysis. This study presents a novel approach for isolating polyethylene terephthalate (PET) and polystyrene (PS) particles and their quantification and identification using Fourier transformation infrared spectroscopy (FTIR) and matrix-assisted laser desorption ionization (MALDI) mass spectrometry. Additionally, seawater samples were analyzed to determine and optimize the microplastic isolation protocol using chemical digestion of the organic matter coupled with density separation of microplastic from sodium metatungstate (SPT) saline solution. Chemical digestion of the organic matter from seawater samples was tested with both hydrogen chloride (HCl) and hydrogen peroxide (H2O2) solutions. FTIR was later used to determine minimal concentration of PET and PS in seawater needed for their identification. Acquired minimal concentration was further tested with reagents used in chemical digestion to assess the effect of various solutions on PET and PS particles identification. The MALDI method for PET and PS identification was used to study the effect of different polymer suspension concentrations and two different cationization agents, sodium iodide (NaI) and silver trifluoroacetate (AgTFA). Identification of both PS and PET with FTIR in different solutions as well as the development of the MALDI method for PET identification were successful.
Abstract (croatian)
Polimeri su makromolekule koje sadrže ponavljajuće strukturne jedinice. Sintetički polimeri su se prvi puta pojavili sredinom 19. stoljeća te od tada njihova proizvodnja eksponencijalno raste na godišnjoj razini. Od 2015. godine, više od 6300 Mt plastičnog otpada je nastalo diljem svijeta, s više od 76% od toga otpada odbačenog na odlagališta smeća i u mora. Kako bi u potpunosti mogli procijeniti učinak ovog zagađenja na naš okoliš i nas same, jednostavni standardizirani protokoli bi trebali biti razvijeni za analiziranje čestica mikroplastike. Ovaj rad prikazuje novi postupak u izoliranju čestica polietilen tereftalata (PET) i polistirena (PS) i njihovu identifikaciju pomoću Fourierove transformirane infracrvene spektroskopije (FTIR) i matricom asistirane laserske desorpcije/ionizacije (MALDI) masene spektrometrije. Također, morski uzorci su analizirani kako bi odredili i optimizirali izolacijski protokol koji koristi kemijsku digestiju organskih tvari i razdvajanje mikroplastike temelju gustoće iz slane otopine natrijevog polivolframata (SPT). Kemijska digestija organskih tvari iz uzoraka morske vode je testirana sa klorovodičnom kiselinom (HCl) i vodikovim peroksidom (H2O2). FTIR je potom korišten da bi se odredila minimalna koncentracija PET-a i PS-a u morskoj vodi potrebna za njihovu identifikaciju. Dobivena minimalna koncentracija je potom testirana sa reagensima korištenima u kemijskoj digestiji da bi se provjerio učinak različitih otopina na identifikaciju čestica PET-a i PS-a. Korištena MALDI metoda za identifikacija PET-s i PS-a usporedila je utjecaj različitih koncentracija polimera u otopini i dvaju različitih kationizacijskih agensa, natrijevog jodida (NaI) i srebrovog trifluoroacetata (AgTFA), na rezultate analize. Identifikacija PS-a i PET-a sa FTIR-om u različitim otopinama i razvoj MALDI metode za identifikaciju PET-a su bili uspješni.
Keywords
Keywords (english)
Languageenglish
URN:NBNurn:nbn:hr:193:669565
ProjectNumber: KK.05.1.1.02.0017
Title: Računalni model strujanja, poplavljivanja i širenja onečišćenja u rijekama i obalnim morskim područjima
Title: Computational models of flow, flooding and pollution dispersion in rivers and coastal waters
Acronym: KLIMOD
Leader: Lado Kranjčević
Jurisdiction: Croatia
Funder: EK
Funding stream: EFRR
Study programmeTitle: Medicinal chemistry
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra medicinske kemije (magistar/magistra medicinske kemije)
Type of resourceText
File originBorn digital
Access conditionsEmbargoed access
Embargo expiration date: 2027-07-27
Terms of useLicence In copyright icon
Created on2022-07-27 07:44:10