prikaz prve stranice dokumenta Application of nanotechnologies for quantification measurement of nanoparticles from human cerebrospinal fluid: comparison of atomic force microscopy and tunable resistive pulse sensing
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master's thesis
Application of nanotechnologies for quantification measurement of nanoparticles from human cerebrospinal fluid: comparison of atomic force microscopy and tunable resistive pulse sensing
2021. urn:nbn:hr:193:956840
Vidović, Ivona
University of Rijeka
Faculty of Biotechnology and Drug Development

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Vidović, I. (2021). Application of nanotechnologies for quantification measurement of nanoparticles from human cerebrospinal fluid: comparison of atomic force microscopy and tunable resistive pulse sensing (Master's thesis). Rijeka: University of Rijeka. Retrieved from https://urn.nsk.hr/urn:nbn:hr:193:956840

Vidović, Ivona. "Application of nanotechnologies for quantification measurement of nanoparticles from human cerebrospinal fluid: comparison of atomic force microscopy and tunable resistive pulse sensing." Master's thesis, University of Rijeka, 2021. https://urn.nsk.hr/urn:nbn:hr:193:956840

Vidović, Ivona. "Application of nanotechnologies for quantification measurement of nanoparticles from human cerebrospinal fluid: comparison of atomic force microscopy and tunable resistive pulse sensing." Master's thesis, University of Rijeka, 2021. https://urn.nsk.hr/urn:nbn:hr:193:956840

Vidović, I. (2021). 'Application of nanotechnologies for quantification measurement of nanoparticles from human cerebrospinal fluid: comparison of atomic force microscopy and tunable resistive pulse sensing', Master's thesis, University of Rijeka, accessed 10 February 2025, https://urn.nsk.hr/urn:nbn:hr:193:956840

Vidović I. Application of nanotechnologies for quantification measurement of nanoparticles from human cerebrospinal fluid: comparison of atomic force microscopy and tunable resistive pulse sensing [Master's thesis]. Rijeka: University of Rijeka; 2021 [cited 2025 February 10] Available at: https://urn.nsk.hr/urn:nbn:hr:193:956840

I. Vidović, "Application of nanotechnologies for quantification measurement of nanoparticles from human cerebrospinal fluid: comparison of atomic force microscopy and tunable resistive pulse sensing", Master's thesis, University of Rijeka, Rijeka, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:193:956840

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TitleApplication of nanotechnologies for quantification measurement of nanoparticles from human cerebrospinal fluid: comparison of atomic force microscopy and tunable resistive pulse sensing
Title (croatian)Primjena nanotehnologija za mjerenje koncentracije i promjera izvanstaničnih vezikula iz likvora čovjeka: usporedba mikroskopije atomskih sila i opažanja pomoću podesivog otpornog pulsa
AuthorIvona Vidović
MentorMladenka Malenica (mentor)
MentorMarko Perčić (komentor)
Committee memberKristina Grabušić (član povjerenstva)
Committee memberMirela Sedić (predsjednik povjerenstva)
GranterUniversity of Rijeka
(Faculty of Biotechnology and Drug Development)
Rijeka
Defense date and country2021-09-01, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Engineering
Abstract
Extracellular vesicles (EVs) are membranous nanoparticles that are currently intensively studied for their qualitative and quantitative changes in pathophysiological conditions. Namely, EVs are secreted by practically all cells and their molecular composition reflects the type and state of originating cells. However, research of EVs is hampered due to lacking methods for precise and reliable quantification. The goal of this study was to improve EV size and concentration measurement in their natural environment by applying novel technologies able to directly measure individual nanoparticles. Such quantification is obtained by Tunable Resistive Pulse Sensing (TRPS) and Atomic Force Microscopy (AFM). The size and concentration of EVs were determined in cerebrospinal fluid (CSF) from patients with severe traumatic brain injury, a condition in which EVs have been previously described to change their physical properties. TRPS measurements of the CSF sample showed a mean EV diameter of 64.3 ± 30.7 nm and a concentration of 1.3 x 10^9 particles/mL. The same CSF sample was used for the AFM measurement for which a novel protocol was introduced in tapping mode to obtain images in a liquid environment. Particle diameter distribution was calculated from 10 1x1 µm images containing 170 nanoparticles, resulting in a mean diameter of 59.2 ± 22.1 nm, and a concentration approximation of 1.7 x 10^10 particles/mL. Taken together, both nanotechnologies resulted in similar EV diameter values of around 60 nm. Yet, TRPS brings a quantitative advantage in a simultaneous measurement of EV size and concentration. This research will further contribute to the characterisation of quantitative changes of nanoparticles in biofluids.
Abstract (croatian)
Izvanstanične vezikule (IV) su membranske nanočestice koje se trenutno intenzivno proučavaju zbog njihove kvalitativne i kvantitativne promjene u patofiziološkim stanjima. Naime, IV-e izlučuju praktički sve stanice, a njihov molekularni sastav odražava vrstu i stanje stanica iz kojih potječu. Međutim, istraživanje IV-a je otežano zbog nedostatka metoda za preciznu i pouzdanu kvantifikaciju. Cilj istraživanja bio je poboljšati mjerenje veličine i koncentracije IV-a u njihovom prirodnom okruženju primjenom novih tehnologija koje mogu izravno mjeriti pojedinačne nanočestice. Takva se kvantifikacija dobiva opažanjem pomoću podesivog otpornog pulsa (engl. Tunable Resistive Pulse Sensing, TRPS) i mikroskopijom atomske sile (engl. Atomic Force Microscopy, AFM). Utvrđena je veličina i koncentracija IV-a iz likvora pacijenata s teškom ozljedom mozga, stanju u kojem su IV-e već opisane kako mijenjaju svoja fizička svojstva. TRPS mjerenja uzorka likvora pokazala su srednji promjer IV-a od 64,3 ± 30,7 nm i koncentraciju od 1,3 x 10^9 čestica/ml. Isti uzorak likvora korišten je za mjerenje AFM-om za koje je uveden novi protokol u načinu tapkanja kako bi se dobile slike IV-a u tekućem okruženju. Raspodjela promjera čestica izračunata je iz 10 slika veličine 1x1 µm s ukupno 170 nanočestica, što je rezultiralo srednjim promjerom od 59,2 ± 22,1 nm i približnom koncentracijom od 1,7 x 10^10 čestica/ml. Sveukupno, obje nanotehnologije rezultirale su sličnim vrijednostima promjera IV-a od oko 60 nm. Ipak, TRPS donosi kvantitativnu prednost u istodobnom mjerenju veličine i koncentracije IV-a. Ovo će istraživanje dodatno doprinijeti karakterizaciji kvantitativnih promjena nanočestica u biotekućinama.
Keywords
Keywords (croatian)
Languageenglish
URN:NBNurn:nbn:hr:193:956840
Study programmeTitle: Medicinal chemistry
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra medicinske kemije (magistar/magistra medicinske kemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2021-09-06 21:42:17