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undergraduate thesis
Biotinilacija protutijela i njihova primjena u protočnoj citometriji
2018. urn:nbn:hr:193:167372
Bašić, Ana
University of Rijeka
Faculty of Biotechnology and Drug Development

Cite this document

Bašić, A. (2018). Biotinilacija protutijela i njihova primjena u protočnoj citometriji (Undergraduate thesis). Rijeka: University of Rijeka. Retrieved from https://urn.nsk.hr/urn:nbn:hr:193:167372

Bašić, Ana. "Biotinilacija protutijela i njihova primjena u protočnoj citometriji." Undergraduate thesis, University of Rijeka, 2018. https://urn.nsk.hr/urn:nbn:hr:193:167372

Bašić, Ana. "Biotinilacija protutijela i njihova primjena u protočnoj citometriji." Undergraduate thesis, University of Rijeka, 2018. https://urn.nsk.hr/urn:nbn:hr:193:167372

Bašić, A. (2018). 'Biotinilacija protutijela i njihova primjena u protočnoj citometriji', Undergraduate thesis, University of Rijeka, accessed 19 January 2025, https://urn.nsk.hr/urn:nbn:hr:193:167372

Bašić A. Biotinilacija protutijela i njihova primjena u protočnoj citometriji [Undergraduate thesis]. Rijeka: University of Rijeka; 2018 [cited 2025 January 19] Available at: https://urn.nsk.hr/urn:nbn:hr:193:167372

A. Bašić, "Biotinilacija protutijela i njihova primjena u protočnoj citometriji", Undergraduate thesis, University of Rijeka, Rijeka, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:193:167372

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Metadata
TitleBiotinilacija protutijela i njihova primjena u protočnoj citometriji
Title (english)Antibody biotinylation and its application in flow cytometry
AuthorAna Bašić
MentorVanda Juranić Lisnić (mentor)
Committee memberVanda Juranić Lisnić (predsjednik povjerenstva)
GranterUniversity of Rijeka
(Faculty of Biotechnology and Drug Development)
Rijeka
Defense date and country2018-09-17, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Protutijela nalaze široku primjenu u medicini i biotehnološkim istraživanjima zbog sposobnosti specifičnog vezanja na različite molekule (antigene). Mogu se koristiti u detekciji specifičnih antigena za dijagnozu bolesti, u terapiji imunodeficijencija, raka ili zaraznih bolesti te u raznim biomedicinskim istraživačkim i dijagnostičkim tehnikama: Western blot, imunohistokemija, protočna citometrija, itd. U kliničkoj se praksi sve više radi na stvaranju monoklonskih protutijela koja su mnogostruko učinkovitija i specifičnija od standardnih poliklonskih antiseruma. Kako bi se olakšala njihova detekcija u protočnoj citometriji, protutijela se obilježavaju fluorokromima ili biotinom. U ovom radu biotinom je obilježeno protutijelo anti-m04.17 koje prepoznaje m04 protein mišjeg citomegalovirusa (MCMV) te je ispitana kvaliteta i primjenjivost ovako obilježenog protutijela za detekciju MCMV-om inficiranih stanica u protočnoj citometriji. Dobiveni rezultati pokazali su da je biotinilacija uspješno provedena, da nije uzrokovala uništenje paratopa antitijela te da je protutijelo anti-m04 specifično za m04 protein MCMV-a.
Abstract (english)
Antibodies have a wide use in medicine and biotechnological research due to their ability to bind various molecules (antigens) with high specificity. They can be used for detecting specific antigens for diagnosis of diseases, treatment of some immune deficiencies, cancer, infectious diseases and in different biomedical techniques: Western blotting, immunohistochemistry, flow citometry, etc. Monoclonal antibodies are of special interest for clinical practice since they are more efficient and have higher specificity compared to standard polyclonal antiserums. Antibodies can be labeled with fluorochromes or biotin to make it easier for detection in flow citometry. In this work antibody anti-m04.17 that recognizes m04 protein of mouse cytomegalovirus (MCMV) was labeled with biotin. After labeling, the quality and applicability of such labeled antibody for detection of cells infected with MCMC in flow citometry was tested. The results have shown that biotinylation was succesfull and did not cause the destruction of antibody's paratope and that the antibody anti-m04 is specific for m04 protein of MCMV.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:193:167372
Study programmeTitle: Biotechnology and drug research
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica biotehnologije i istraživanja lijekova (sveučilišni/a prvostupnik/prvostupnica biotehnologije i istraživanja lijekova)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2018-11-06 07:36:07