prikaz prve stranice dokumenta Analiza stanica NK kod CD16 deficitnih životinja
Embargo period 2030-10-23
master's thesis
Analiza stanica NK kod CD16 deficitnih životinja
2024. urn:nbn:hr:193:658692
Lačić, Laura
University of Rijeka
Faculty of Biotechnology and Drug Development

Cite this document

Lačić, L. (2024). Analiza stanica NK kod CD16 deficitnih životinja (Master's thesis). Rijeka: University of Rijeka. Retrieved from https://urn.nsk.hr/urn:nbn:hr:193:658692

Lačić, Laura. "Analiza stanica NK kod CD16 deficitnih životinja." Master's thesis, University of Rijeka, 2024. https://urn.nsk.hr/urn:nbn:hr:193:658692

Lačić, Laura. "Analiza stanica NK kod CD16 deficitnih životinja." Master's thesis, University of Rijeka, 2024. https://urn.nsk.hr/urn:nbn:hr:193:658692

Lačić, L. (2024). 'Analiza stanica NK kod CD16 deficitnih životinja', Master's thesis, University of Rijeka, accessed 19 January 2025, https://urn.nsk.hr/urn:nbn:hr:193:658692

Lačić L. Analiza stanica NK kod CD16 deficitnih životinja [Master's thesis]. Rijeka: University of Rijeka; 2024 [cited 2025 January 19] Available at: https://urn.nsk.hr/urn:nbn:hr:193:658692

L. Lačić, "Analiza stanica NK kod CD16 deficitnih životinja", Master's thesis, University of Rijeka, Rijeka, 2024. Available at: https://urn.nsk.hr/urn:nbn:hr:193:658692

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Metadata
TitleAnaliza stanica NK kod CD16 deficitnih životinja
Title (english)Analysis of NK cells in CD16 deficient animals
AuthorLaura Lačić
MentorVedrana Jelenčić (mentor)
Committee memberMladen Merćep (predsjednik povjerenstva)
Committee memberDaniela Kalafatović (član povjerenstva)
GranterUniversity of Rijeka
(Faculty of Biotechnology and Drug Development)
Rijeka
Defense date and country2024-10-23, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Molecular Biotechnology
Abstract
Prirodnoubilačke ili stanice NK (engl. natural killer cells, NK cells) važna su sastavnica urođenog imunološkog sustava zbog svoje sposobnosti brzog prepoznavanja i eliminacije tumorskih i virusom zaraženih stanica. Njihova aktivacija ovisi o ravnoteži signala primljenih preko aktivacijskih i inhibicijskih receptora. Jedan od aktivacijskih receptora izraženih na stanicama NK je i CD16 receptor, poznat po svojoj ulozi u staničnoj citotoksičnosti posredovanoj protutijelima (engl. antibody-dependent cellular cytotoxicity, ADCC). Međutim, uloga ovog receptora u drugim aspektima biologije stanica NK još uvijek nije dovoljno istražena. Stoga je naš glavni cilj bio ispitati utjecaj nedostatka izražaja CD16 receptora na stanice NK u različitim organima. Koristili smo genetski modificirane miševe kojima nedostaje izražaj CD16 receptora i usporedili ih s miševima divljeg soja. Proveli smo fenotipsku i funkcionalnu analizu stanica NK. Uočili smo da nedostatak izražaja CD16 receptora dovodi do promjene u sazrijevanju stanica NK, kao i do promjena u njihovom postotku i broju u koštanoj srži. Nedostatak izražaja ovog receptora nije doveo do promjene u izražaju većine analiziranih aktivacijskih i inhibicijskih receptora, izuzev NCR1 receptora čiji je izražaj smanjen kod CD16 deficitnih životinja. Također, CD16 deficitne stanice NK nisu pokazale razliku u sposobnosti produkcije citokina u odnosu na kontrolnu grupu. Rezultati ovoga rada ukazuju na dosad nepoznatu ulogu CD16 receptora u sazrijevanju stanica NK iako su potrebna dodatna istraživanja kako bi se ta uloga do kraja istražila.
Abstract (english)
Natural killer (NK) cells are an important component of the innate immune system due to their ability to rapidly recognize and eliminate tumor cells and virus-infected cells. Their activation depends on a balance of signals received through activating and inhibitory receptors. One key activating receptor expressed on NK cells is the CD16 receptor, known for its role in antibody-dependent cellular cytotoxicity (ADCC). However, the role of this receptor in other aspects of NK cell biology remains largely unexplored. The main aim of this study was to investigate the impact of CD16 receptor deficiency on NK cells in different organs. We used genetically modified mice lacking CD16 expression and compared them to wild-type mice. We performed phenotypic and functional analyses of NK cells. We observed that the absence of CD16 expression leads to changes in NK cell maturation, as well as changes in their percentage and number in the bone marrow. The absence of this receptor did not result in changes in the expression of most analysed activating and inhibitory receptors, except for the NCR1 receptor, which showed reduced expression in CD16-deficient animals. Additionally, CD16-deficient NK cells did not show differences in cytokine production capacity compared to the control group. These findings indicate a previously unknown role of the CD16 receptor in NK cell maturation, although further studies are needed to fully elucidate this role.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:193:658692
Study programmeTitle: Biotechnology in medicine
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra biotehnologije u medicini (magistar/magistra biotehnologije u medicini)
Type of resourceText
File originBorn digital
Access conditionsEmbargoed access
Embargo expiration date: 2030-10-23
Terms of useLicence In copyright icon
Created on2024-10-22 17:48:06