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master's thesis
Vizualizacija proliferirajućih imunosnih stanica u mišjem modelu metabolički uzrokovane bolesti masne jetre (MASLD)
2024. urn:nbn:hr:193:472123
Plantak, Patricia
University of Rijeka
Faculty of Biotechnology and Drug Development

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Plantak, P. (2024). Vizualizacija proliferirajućih imunosnih stanica u mišjem modelu metabolički uzrokovane bolesti masne jetre (MASLD) (Master's thesis). Rijeka: University of Rijeka. Retrieved from https://urn.nsk.hr/urn:nbn:hr:193:472123

Plantak, Patricia. "Vizualizacija proliferirajućih imunosnih stanica u mišjem modelu metabolički uzrokovane bolesti masne jetre (MASLD)." Master's thesis, University of Rijeka, 2024. https://urn.nsk.hr/urn:nbn:hr:193:472123

Plantak, Patricia. "Vizualizacija proliferirajućih imunosnih stanica u mišjem modelu metabolički uzrokovane bolesti masne jetre (MASLD)." Master's thesis, University of Rijeka, 2024. https://urn.nsk.hr/urn:nbn:hr:193:472123

Plantak, P. (2024). 'Vizualizacija proliferirajućih imunosnih stanica u mišjem modelu metabolički uzrokovane bolesti masne jetre (MASLD)', Master's thesis, University of Rijeka, accessed 05 January 2025, https://urn.nsk.hr/urn:nbn:hr:193:472123

Plantak P. Vizualizacija proliferirajućih imunosnih stanica u mišjem modelu metabolički uzrokovane bolesti masne jetre (MASLD) [Master's thesis]. Rijeka: University of Rijeka; 2024 [cited 2025 January 05] Available at: https://urn.nsk.hr/urn:nbn:hr:193:472123

P. Plantak, "Vizualizacija proliferirajućih imunosnih stanica u mišjem modelu metabolički uzrokovane bolesti masne jetre (MASLD)", Master's thesis, University of Rijeka, Rijeka, 2024. Available at: https://urn.nsk.hr/urn:nbn:hr:193:472123

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Metadata
TitleVizualizacija proliferirajućih imunosnih stanica u mišjem modelu metabolički uzrokovane bolesti masne jetre (MASLD)
Title (english)Visualization of proliferating immune cells in a mouse model of metabolic associated steatotic liver disease (MASLD)
AuthorPatricia Plantak
MentorMaja Lenartić (mentor)
MentorJelena Ban (komentor)
Committee memberStribor Marković (predsjednik povjerenstva)
Committee memberDean Marković (član povjerenstva)
Committee memberMaja Lenartić (član povjerenstva)
Committee memberJelena Ban (član povjerenstva)
GranterUniversity of Rijeka
(Faculty of Biotechnology and Drug Development)
Rijeka
Defense date and country2024-09-26, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Biology
Abstract
Moderni način života često dovodi do stresa, neaktivnosti i manjka sna, što rezultira lošim prehrambenim navikama bogatim rafiniranim šećerima i zasićenim mastima. Dugotrajna izloženost takvom stilu života može dovesti do povišenog kolesterola, triglicerida, šećera u krvi, inzulinske rezistencije i visokog krvnog tlaka, što se zajednički naziva metaboličkim sindromom. Metabolički uzrokovana bolest steatotične jetre (engl. metabolic dysfunction-associated steatotic liver disease, MASLD) predstavlja manifestaciju ovog sindroma, a obilježava je nakupljanje masti u jetri. MASLD napreduje kroz različite faze, od steatoze, preko fibroze pa sve do ciroze. Opći je konsenzus da aktivacija proupalnih imunosnih efektora igra ključnu ulogu u tranziciji steatoze u steatohepatitis uzrokovan metaboličkom disfunkcijom (engl. metabolic dysfunction-associated steatohepatitis, MASH). Cilj diplomskog rada bio je dokazati da zapadnjačka prehrana kod miševa dovodi do razvoja MASH-a, prvenstveno infiltracijom aktivnih proliferirajućih imunosnih stanica u jetri. Korištenjem imunohistokemijskih i imunofluorescentnih metoda identificirali smo te stanice, dok je protočna citometrija omogućila detaljnu analizu njihovih podtipova. U miševa hranjenih prehranom SSD, otkrili smo histopatološke promjene u jetri koje odgovaraju onima u MASH pacijenata. Imunohistokemijska bojenja pokazala su prisutnost CD45+ i Ki-67+ imunosnih stanica u tkivu jetre, dok je imunofluorescencija potvrdila dvostruko pozitivne stanice. Kod miševa na SSD prehrani primijetili smo povećanu infiltraciju imunosnih stanica, posebno unutar upalnih žarišta, s većim brojem CD45+Ki-67+ stanica. Također, rezultati protočne citometrije pokazali su značajan porast proliferirajućih CD8+ limfocita T, NK stanica i γδ limfocita T u jetrama SSD miševa u usporedbi s kontrolnom grupom što ukazuje na aktivnu upalu u jetri uzrokovanu SSD prehranom. Ove promjene sugeriraju da prehrana SSD značajno utječe na proliferaciju i dinamiku leukocita u tkivu jetre, potencijalno mijenjajući imunosni odgovor i pridonoseći progresiji MASLD-a. Ovi rezultati mogli bi usmjeriti buduća istraživanja na imunološke aspekte bolesti, što bi pomoglo u razvoju novih dijagnostičkih i terapijskih strategija za MASLD.
Abstract (english)
The modern lifestyle often leads to stress, inactivity, and lack of sleep, resulting in poor eating habits rich in refined sugars and saturated fats. Prolonged exposure to this lifestyle can cause elevated cholesterol, triglycerides, blood sugar, insulin resistance, and high blood pressure, which are collectively referred to as metabolic syndrome. Metabolic dysfunction-associated steatotic liver disease (MASLD) is a manifestation of this syndrome and is characterized by fat accumulation in the liver. MASLD includes various stages, ranging from steatosis to cirrhosis. It is widely accepted that the activation of pro-inflammatory immune effectors plays a key role in the transition from steatosis to metabolic dysfunction-associated steatohepatitis (MASH). This thesis aimed to demonstrate that a Western diet in mice leads to the development of MASH, primarily through the infiltration of actively proliferating immune cells into the liver. Using immunohistochemical and immunofluorescent methods, these cells were identified, while flow cytometry provided detailed analysis of their subtypes. In SSD-fed mice, we discovered histopathological changes in the liver resembling those observed in MASH patients. Immunohistochemical staining revealed the presence of CD45+ and Ki-67+ immune cells in the liver tissue, and immunofluorescence confirmed the presence of double-positive cells. In SSD-fed mice, we observed increased immune cell infiltration, particularly within inflammatory foci, with a higher number of CD45+Ki-67+ cells. Additionally, flow cytometry results showed a significant increase in proliferating CD8+ T lymphocytes, NK cells and γδ T lymphocytes in the livers of SSD-fed mice compared to the control group indicating active liver inflammation caused by the SSD diet. These changes suggest that the SSD diet significantly affects leukocyte proliferation and dynamics within liver, potentially altering the immune response and contributing to the progression of MASLD. These findings may direct future research toward the immunological aspects of the disease, aiding in the development of new diagnostic and therapeutic strategies for MASLD.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:193:472123
Study programmeTitle: Biotechnology in medicine
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra biotehnologije u medicini (magistar/magistra biotehnologije u medicini)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2024-09-23 00:23:51