prikaz prve stranice dokumenta Voltammetric and spectroscopic studies of DNA interaction with riboflavin, daunorubicin and doxorubicin
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master's thesis
Voltammetric and spectroscopic studies of DNA interaction with riboflavin, daunorubicin and doxorubicin
2017. urn:nbn:hr:193:126790
Marinović, Iva
University of Rijeka
Faculty of Biotechnology and Drug Development
Poland: University of Warsaw

Cite this document

Marinović, I. (2017). Voltammetric and spectroscopic studies of DNA interaction with riboflavin, daunorubicin and doxorubicin (Master's thesis). Rijeka: University of Rijeka; Varšava: Poland: University of Warsaw. Retrieved from https://urn.nsk.hr/urn:nbn:hr:193:126790

Marinović, Iva. "Voltammetric and spectroscopic studies of DNA interaction with riboflavin, daunorubicin and doxorubicin." Master's thesis, University of Rijeka; Poland: University of Warsaw, 2017. https://urn.nsk.hr/urn:nbn:hr:193:126790

Marinović, Iva. "Voltammetric and spectroscopic studies of DNA interaction with riboflavin, daunorubicin and doxorubicin." Master's thesis, University of Rijeka; Poland: University of Warsaw, 2017. https://urn.nsk.hr/urn:nbn:hr:193:126790

Marinović, I. (2017). 'Voltammetric and spectroscopic studies of DNA interaction with riboflavin, daunorubicin and doxorubicin', Master's thesis, University of Rijeka; Poland: University of Warsaw, accessed 12 December 2024, https://urn.nsk.hr/urn:nbn:hr:193:126790

Marinović I. Voltammetric and spectroscopic studies of DNA interaction with riboflavin, daunorubicin and doxorubicin [Master's thesis]. Rijeka: University of Rijeka; Varšava: Poland: University of Warsaw; 2017 [cited 2024 December 12] Available at: https://urn.nsk.hr/urn:nbn:hr:193:126790

I. Marinović, "Voltammetric and spectroscopic studies of DNA interaction with riboflavin, daunorubicin and doxorubicin", Master's thesis, University of Rijeka, Rijeka; Poland: University of Warsaw, Varšava, 2017. Available at: https://urn.nsk.hr/urn:nbn:hr:193:126790

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Metadata
TitleVoltammetric and spectroscopic studies of DNA interaction with riboflavin, daunorubicin and doxorubicin
Title (croatian)Voltametrijske i spektroskopske studije interakcije DNA s riboflavinom, daunorubicinom i doksorubicinom
AuthorIva Marinović
MentorMagdalena Maj-Zurawska (mentor)
MentorKatja Džepina (mentor)
Committee memberNela Malatesti (predsjednik povjerenstva)
Committee memberDražen Vikić-Topić (član povjerenstva)
Committee memberMagdalena Maj-Zurawska (član povjerenstva)
Committee memberKatja Džepina (član povjerenstva)
GranterUniversity of Rijeka
(Faculty of Biotechnology and Drug Development)
Rijeka
GranterPoland: University of Warsaw
Varšava
Defense date and country2017-10-27, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Riboflavin (RF), vitamin B2 and anthracyclines, daunorubicin (DAU) and doxorubicin (DOX) showed the influence on electrochemical and spectral properties of the DNA molecule. The interactions were analysed using voltammetry, UV/VIS absorbance spectrophotometry and circular dichroism spectroscopy. These interactions were investigated by monitoring changes: in electrochemical properties of ctDNA and oligonucleotides interacting with RF on the surface of screen-printed carbon electrode (SPCE) surface using square-wave voltammetry (SWV), in UV/VIS absorption spectra of ctDNA, oligonucleotides and RF and in circular dichroism spectra of DNA in increasing concentration of RF and anthracyclines. The obtained results indicate that interactions between investigated compounds and DNA depend on the DNA sequence and the concentration of ligand. As the result of interactions, oxidation signals of guanine and adenine, absorbance spectra of DNA and RF, and CD spectra of oligonucleotides changes. RF binding to DNA causes decrease in number of exposed oxidative functional groups proved by decrease in oxidation signal of bases and lowered absorbance signal of DNA and RF mixture. CD investigations showed the influence of RF and anthracyclines on the DNA conformation. RF showed preference on AT regions while anthracyclines bind on different way in different DNA regions depending on concentration. At lower concentrations, they intercalate to GC and bind to the groove of AT regions while higher concentration induces conformational changes to Z-DNA.
Abstract (croatian)
Riboflavin, vitamin B2 i antraciklini, daunorubicin (DAU) i doksorubicin (DOX) su pokazali utjecaj na elektrokemijska i spektralna svojstva DNA molekule. Interakcije su analizirane pomoću voltametrije, UV/VIS apsorpcijske spektrofotometrije i cirkularni dikroizam spektroskopije. Interakcije su analizirane praćenjem promjena u: elektrokemijskim svojstvima ctDNA i oligonukleotida u interakciji s RF na površini tiskane ugljikove electrode (SPCE) upotrebom kvadratno-valne voltametrije (SWV), UV/VIS apsorpcijskim spektrima ctDNA, oligonukleotida i RF te DNA spektru cirkularnog dikroizma u povećanju koncentracije RF i antraciklina. Dobiveni rezultati pokazuju da interakcije između DNA i ispitivanih spojeva ovise o slijedu DNA i koncentraciji liganda. Kao rezultat interakcija, mijenjaju se oksidacijski signali gvanina i adenine, apsorpcijski spektri DNA i RF te CD spektri oligonukleotida. Vezanje RF na DNA uzrokuje smanjenje broja izloženih oksidacijskih funkcionalnih skupina što je dokazano smanjenjem oksidacijskih signala baza te sniženim apsorcijskim signalom smjese DNA i RF. CD ispitivanja su pokazala utjecaj RF i antraciklina na DNA konformaciju. RF je pokazao veći afinitet prema AT regijama dok se antraciklini vežu na različite načine u različitim područjima DNA, ovisno o koncentraciji. Pri nižim koncentracijama, interkaliraju se na GC i vežu na utor AT regije, dok veća koncentracija izaziva konformacijske promjene u Z-DNA.
Keywords
Keywords (croatian)
Languageenglish
URN:NBNurn:nbn:hr:193:126790
Study programmeTitle: Drug research and development
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra istraživanja i razvoja lijekova (magistar/magistra istraživanja i razvoja lijekova)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2018-01-15 09:22:25