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undergraduate thesis
Uloga m6A modifikacije u biološkim procesima, bolestima i infekcijama
2024. urn:nbn:hr:193:183045
Petrović, Magdalena
University of Rijeka
Faculty of Biotechnology and Drug Development

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Petrović, M. (2024). Uloga m6A modifikacije u biološkim procesima, bolestima i infekcijama (Undergraduate thesis). Rijeka: University of Rijeka. Retrieved from https://urn.nsk.hr/urn:nbn:hr:193:183045

Petrović, Magdalena. "Uloga m6A modifikacije u biološkim procesima, bolestima i infekcijama." Undergraduate thesis, University of Rijeka, 2024. https://urn.nsk.hr/urn:nbn:hr:193:183045

Petrović, Magdalena. "Uloga m6A modifikacije u biološkim procesima, bolestima i infekcijama." Undergraduate thesis, University of Rijeka, 2024. https://urn.nsk.hr/urn:nbn:hr:193:183045

Petrović, M. (2024). 'Uloga m6A modifikacije u biološkim procesima, bolestima i infekcijama', Undergraduate thesis, University of Rijeka, accessed 25 January 2025, https://urn.nsk.hr/urn:nbn:hr:193:183045

Petrović M. Uloga m6A modifikacije u biološkim procesima, bolestima i infekcijama [Undergraduate thesis]. Rijeka: University of Rijeka; 2024 [cited 2025 January 25] Available at: https://urn.nsk.hr/urn:nbn:hr:193:183045

M. Petrović, "Uloga m6A modifikacije u biološkim procesima, bolestima i infekcijama", Undergraduate thesis, University of Rijeka, Rijeka, 2024. Available at: https://urn.nsk.hr/urn:nbn:hr:193:183045

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Metadata
TitleUloga m6A modifikacije u biološkim procesima, bolestima i infekcijama
Title (english)The role of m6A modification in biological processes, diseases and infections
AuthorMagdalena Petrović
MentorIgor Jurak (mentor)
Committee memberIvan Gudelj (predsjednik povjerenstva)
Committee memberChristian Andrew Reynolds (član povjerenstva)
GranterUniversity of Rijeka
(Faculty of Biotechnology and Drug Development)
Rijeka
Defense date and country2024-09-19, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
N6-metiladenozin (m6A) najčešća je modifikacija eukariotskih RNA. Metilacija adenozina odvija se kotranskripcijski u staničnoj jezgri. Regulirana je proteinima koji metiliraju RNA poput METTL3, METTL14 i WTAP zvanih pisači, proteinima koji demetiliraju RNA poput FTO i ALKHB5 zvanih brisači te proteinima koji uočavaju ovu modifikaciju poput YTHDC1-2, YTHDF1-3 i HNRNPC zvanih čitači. M6A regulira ekspresiju gena tako što utječe na stabilnost, prekrajanje i prijenos mRNA iz jezgre te na njenu razgradnju. Koregulacjia translacije i raspadanja koju provode proteini regulatori m6A modifikacije dovodi do ubrzanog metabolizma mRNA i može dovesti do pojačanja proizvodnje proteina. Regulacija ekspresije gena presudna je pri diferencijaciji stanica pa ne čudi da m6A i tamo igra ključnu ulogu. Međutim, regulatori m6A nisu u svim tkivima podjednako aktivni pa se funkcija m6A najbolje očituje u organima reproduktivnog, živčanog i imunosnog sustava. Upravo zbog tog značajnog utjecaja na imunosni sustav, tumori i patogeni iskorištavaju m6A modifikaciju kako bi smanjili imunosni odgovor te kako bi bolest koju uzrokuju mogla bolje napredovati. Štoviše, neki virusi mogu iskoristiti i regulatore m6A kako bi pojačali ekspresiju svojih gena, a smanjili ekspresiju gena domaćina. Herpes simpleks virus tipa 1 poseban je po tome što uzrokuje premještanje proteina regulatora m6A tijekom infekcije. Virusni protein ICP27 koji regulira preradu RNA uzrokuje premještanje METTL3 i METTL14 iz jezgre u citoplazmu domaćina. Daljnja su istraživanja potrebna kako bi se pojasnili svi mehanizmi djelovanja regulatora m6A modifikacije i njihovog međudjelovanja s virusnim proteinima tijekom infekcije.
Abstract (english)
N6-methyladenosine (m6A) is the most common modification of the eukaryotic RNA. Methylation of adenosine occurs cotranscriptionally in the cell's nucleus. It is regulated by proteins that methylate RNA such as METTL3, METTL14 and WTAP called writers, proteins that demethylate RNA such as FTO and ALKBH5 called erasers and proteins that recognize this modification such as YTHDC1-2, YTHDF1-3 and HNRNPC called readers. M6A regulates gene expression by influencing the stability, splicing and transport of mRNA from the nucleus as well as its decay. The coregulation of translation and decay which is done by YTHDF1 and YTHDF2 causes acceleration of mRNA metabolism and can lead to an increase in protein production. Regulation of gene expression is vital during cell differentiation so it is not surprising that m6A is crucial in that process. However, the activity of m6A regulators differs between tissues and the function of m6A is most easily seen in the reproductive, nervous and immune systems. Because of that significant influence over the immune system, tumors and pathogens use m6A modification to suppress immune response and allow the progression of disease. Herpes simplex virus type 1 causes redistribution of m6A regulators. Viral protein ICP27 which regulates RNA processing causes METTL3 and METTL14 to transfer from the nucleus to the cytoplasm of the host. Further research is needed to clarify the mechanisms of action of m6A regulators and their interaction with viral proteins during infection.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:193:183045
Study programmeTitle: Biotechnology and drug research
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) biotehnologije i istraživanja lijekova (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) biotehnologije i istraživanja lijekova)
Type of resourceText
File originBorn digital
Access conditionsOpen access
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Created on2024-09-17 22:11:44