prikaz prve stranice dokumenta Effect of supplementation with glutamine and choline chloride during isolation on the phenotype of preferential methamphetamine consumption in D. melanogaster
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undergraduate thesis
Effect of supplementation with glutamine and choline chloride during isolation on the phenotype of preferential methamphetamine consumption in D. melanogaster
2024. urn:nbn:hr:193:968074
Cikač, Petra
University of Rijeka
Faculty of Biotechnology and Drug Development

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Cikač, P. (2024). Effect of supplementation with glutamine and choline chloride during isolation on the phenotype of preferential methamphetamine consumption in D. melanogaster (Undergraduate thesis). Rijeka: University of Rijeka. Retrieved from https://urn.nsk.hr/urn:nbn:hr:193:968074

Cikač, Petra. "Effect of supplementation with glutamine and choline chloride during isolation on the phenotype of preferential methamphetamine consumption in D. melanogaster." Undergraduate thesis, University of Rijeka, 2024. https://urn.nsk.hr/urn:nbn:hr:193:968074

Cikač, Petra. "Effect of supplementation with glutamine and choline chloride during isolation on the phenotype of preferential methamphetamine consumption in D. melanogaster." Undergraduate thesis, University of Rijeka, 2024. https://urn.nsk.hr/urn:nbn:hr:193:968074

Cikač, P. (2024). 'Effect of supplementation with glutamine and choline chloride during isolation on the phenotype of preferential methamphetamine consumption in D. melanogaster', Undergraduate thesis, University of Rijeka, accessed 18 January 2025, https://urn.nsk.hr/urn:nbn:hr:193:968074

Cikač P. Effect of supplementation with glutamine and choline chloride during isolation on the phenotype of preferential methamphetamine consumption in D. melanogaster [Undergraduate thesis]. Rijeka: University of Rijeka; 2024 [cited 2025 January 18] Available at: https://urn.nsk.hr/urn:nbn:hr:193:968074

P. Cikač, "Effect of supplementation with glutamine and choline chloride during isolation on the phenotype of preferential methamphetamine consumption in D. melanogaster", Undergraduate thesis, University of Rijeka, Rijeka, 2024. Available at: https://urn.nsk.hr/urn:nbn:hr:193:968074

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Metadata
TitleEffect of supplementation with glutamine and choline chloride during isolation on the phenotype of preferential methamphetamine consumption in D. melanogaster
Title (croatian)Utjecaj suplementacije glutaminom i kolinom kloridom tijekom izolacije na fenotip preferencijalne konzumacije metamfetamina kod D. melanogaster
AuthorPetra Cikač
MentorRozi Andretić Waldowski (mentor)
MentorAna Filošević Vujnović (komentor)
Committee memberToni Todorovski (predsjednik povjerenstva)
Committee memberChristian Andrew Reynolds (član povjerenstva)
Committee memberAna Filošević Vujnović (član povjerenstva)
GranterUniversity of Rijeka
(Faculty of Biotechnology and Drug Development)
Rijeka
Defense date and country2024-09-17, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Ovisnost je globalni problem koji pogađa mnoge ljude i zajednice, zbog čega je važno istražiti molekularne mehanizme ovisnosti. Jedan od glavnih okolišnih stresora, socijalna izolacija, narušava ravnotežu neurokemikalija u mozgu, što posljedično utječe na ponašanja povezana s ovisnošću. Ova studija ispituje učinke dodavanja prekursora neurotransmitera i socijalne izolacije na sklonost metamfetaminu (METH) kod D. melanogaster. U ovoj studiji, divlji tip mušica starih tri do pet dana izoliran je na pet dana uz dohranu s 10 mg/mL L-glutamina (GLU) prekursora glutamata ili 3 mg/mL kolin klorida (CH.CL) prekursora acetilkolina. Nakon izolacije i predtretmana mušice su prebačene u FlyCAFÉ esej, gdje mi je tri uzastopna ponuđen izbor između obične hrane i hrane s dodatkom METH-a. Preferencija za METH-a je izračunata kao razlika između volumena konzumirane METH-hrane i uobičajene hrane za individualnu mušicu, a podaci su prikazani kao prosjek za svaki dan eksperimenta. Rezultati ove studije su pokazali da suplementacija s GLU ili CH.CL tijekom pet dana izolacije povećava preferenciju METH-a u FlyCAFÉ-u, što je suprotno prethodnim studijama gdje je pokazano da izolacija smanjuje preferenciju za METH u odnosu na neizolirane mušice. Ovo istraživanje otkriva da dohrana s GLU ili CH.CL smanjuje učinak izazvan izolacijom, uzimajući u obzir socijalne i biokemijske aspekte u testu preferencije METH kod mušica. Budući genetski testovi trebaju potvrditi ulogu glutamina i acetilkolina u regulaciji ovog endofenotipa ovisnosti kod mušica.
Abstract (english)
Addiction represents a global challenge to the world, affecting many individuals and communities, making it necessary to examine the mechanisms of addiction. One major environmental stressor, social isolation, disrupts the balance of neurochemicals in the brain that subsequently influence the behaviors related to addiction. This study examines the effects of neurotransmitter precursor supplementation and social isolation on methamphetamine (METH) preference in D. melanogaster. Wild-type three to five day-old male flies were isolated for five days and supplemented with either 10 mg/mL L-Glutamine (GLU) precursor of glutamate, or 3 mg/mL choline chloride (CH.CL) precursor of acetylcholine. After isolation and pretreatment, we used the FlyCAFÉ assay where flies were offered a choice between regular food and METH-supplemented food for three consecutive days. METH preference was calculated as difference between METH-food and regular food for induvial fly, and data were presented as average for every day of the experiment. The results of this study have shown that supplementation with GLU or CH.CL during five days of isolation increased METH preference in FlyCAFÉ, what contrasts with previous studies where it was shown that isolation reduces METH preference relative to non-isolated flies. This research reveals that GLU or CH.CL supplementation reduced effect induced by isolation, considering social and biochemical aspects in test for METH preference in flies. Future genetic tests are needed to confirm role of glutamine and acetylcholine in the regulation of this addiction endophenotype in flies.
Keywords
Keywords (english)
Languageenglish
URN:NBNurn:nbn:hr:193:968074
Study programmeTitle: Biotechnology and drug research
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) biotehnologije i istraživanja lijekova (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) biotehnologije i istraživanja lijekova)
Type of resourceText
File originBorn digital
Access conditionsOpen access
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Created on2024-09-12 16:34:45